modified_mpob_medium (CultureMech:007559)

Overview

External ontology

TOGO:M1043 — Modified MPOB Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M1043 Original source: JCM - JCM_M990 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=990

Composition

flowchart LR
    CultureMech_007559["modified_mpob_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007559 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007559 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007559 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007559 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007559 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_007559 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007559 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Na2HPO4["Na2HPO4"]
    CultureMech_007559 --> ing7_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing7_Na2HPO4 -.-> CHEBI_34683
    ing8_Glucose["Glucose"]
    CultureMech_007559 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007559 --> ing9_Yeast_extract__BD_Difco_
    ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007559 --> ing10_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007559 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007559 --> ing12_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing12_Nitrogen_gas -.-> CHEBI_17997

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	NaCl Cl.Na 58.443 g/mol	0.3 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.11 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.41 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	NH4Cl Cl.H4N 53.492 g/mol	0.3 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6	Resazurin C12H7NO4 229.191 g/mol	0.5 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
7	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.1 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8	Na2HPO4 HO4P.2Na 141.958 g/mol	0.53 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
9	Glucose C6H12O6 180.156 g/mol	1.8 G_PER_L	CHEBI:17234 glucose	Role: Carbon source; Properties: Defined component, Simple component, Organic compound
10	Yeast extract (BD-Difco)	0.4 G_PER_L		Properties: Undefined component
11	L--Cysteine・HCl・H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.5 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Properties: Defined component, Simple component, Organic compound
12	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
13	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス

Solutions

Trace element solution SL--10 (see Medium [M433])

Dose: 1 G_PER_L

Cross-reference to Medium M433

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:53.815130Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1043
2026-01-28T19:48:36.216794Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:00.370912+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T06:19:49.663919Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007559
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:30:42.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.011615+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified MPOB Medium" to "modified_mpob_medium" for consistent matching
2026-05-16T09:03:07.137513+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:29.958419+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.780279+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.