Overview
- External ontology
- TOGO:M1046 — Diluted Nitrate Mineral Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1046 Original source: JCM - JCM_M992-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=992
Composition
flowchart LR
CultureMech_007562["diluted_nitrate_mineral_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007562 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007562 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007562 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KNO3["KNO3"]
CultureMech_007562 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_agar["agar"]
CultureMech_007562 --> ing4_agar
CHEBI_2509["CHEBI:2509"]
ing4_agar -.-> CHEBI_2509
ing5_methane["methane"]
CultureMech_007562 --> ing5_methane
CHEBI_16183["CHEBI:16183"]
ing5_methane -.-> CHEBI_16183
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
990 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
0.2 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 |
methane
CH4 16.043 g/mol
|
variable VARIABLE |
CHEBI:16183
methane
|
Properties: Gas |
Solutions
Modified SL--4 trace element solution (see Medium [M1045])
Dose: 1 G_PER_L
Cross-reference to Medium M1045
Chelated iron solution (see Medium [M863])
Dose: 10 G_PER_L
Cross-reference to Medium M863
0.2 M KH2PO4 solution
Dose: 5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
0.2 M Na2HPO4 solution
Dose: 5 G_PER_L
Role: Buffer; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.820749Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1046 |
| 2026-01-28T19:48:43.363479Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: methane |
| 2026-03-09T08:05:13.154695+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:49.687942Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007562 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.037489+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Diluted Nitrate Mineral Medium" to "diluted_nitrate_mineral_medium" for consistent matching |
| 2026-05-16T09:03:07.165871+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:29.988055+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.887715+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-20T19:01:16.507447+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |