m30

CultureMech:007565 bacterial COMPLEX LIQUID
Original name: M30

Overview

External ontology
TOGO:M1049 — M30
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1049 Original source: JCM - JCM_M995 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=995

Composition

flowchart LR
    CultureMech_007565["m30"]
    ing0_0_1_M_Tris__HCl_buffer__pH_7_5_["0.1 M Tris--HCl buffer (pH 7.5)"]
    CultureMech_007565 --> ing0_0_1_M_Tris__HCl_buffer__pH_7_5_
    ing1_Distilled_water["Distilled water"]
    CultureMech_007565 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Aged_seawater["Aged seawater"]
    CultureMech_007565 --> ing2_Aged_seawater
    ing3_Ampicillin__50_mg_ml_["Ampicillin (50 mg/ml)"]
    CultureMech_007565 --> ing3_Ampicillin__50_mg_ml_
    ing4_Na2HPO4_2H2O["Na2HPO4・2H2O"]
    CultureMech_007565 --> ing4_Na2HPO4_2H2O
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4_2H2O -.-> CHEBI_34683
    ing5_N__Acetyl__D__glucosamine__Sigma_["N--Acetyl--D--glucosamine (Sigma)"]
    CultureMech_007565 --> ing5_N__Acetyl__D__glucosamine__Sigma_
    ing6_Biotin["Biotin"]
    CultureMech_007565 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
    CultureMech_007565 --> ing7_p__Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing7_p__Aminobenzoic_acid -.-> CHEBI_30753
    ing8_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007565 --> ing8_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing8_Calcium_pantothenate -.-> CHEBI_31345
    ing9_Pyridoxine_HCl["Pyridoxine・HCl"]
    CultureMech_007565 --> ing9_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing9_Pyridoxine_HCl -.-> CHEBI_30961
    ing10_Folic_acid["Folic acid"]
    CultureMech_007565 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_007565 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_Riboflavin["Riboflavin"]
    CultureMech_007565 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_Nicotinamide["Nicotinamide"]
    CultureMech_007565 --> ing13_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing13_Nicotinamide -.-> CHEBI_17154
    ing14_Thiamine_HCl_2H2O["Thiamine・HCl・2H2O"]
    CultureMech_007565 --> ing14_Thiamine_HCl_2H2O

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 0.1 M Tris--HCl buffer (pH 7.5) 50 G_PER_L Role: Buffer; Properties: Complex component, Defined component, Solution
2 Distilled water
H2O 18.015 g/mol
301.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 260.0, 40.0, 1.0]
3 Aged seawater 700 G_PER_L Role: Solvating media; Properties: Undefined component, Complex component
4 Ampicillin (50 mg/ml) 4 G_PER_L Role: Antimicrobial agent; Properties: Defined component, Organic compound, Solution
5 Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
0.01 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 N--Acetyl--D--glucosamine (Sigma) 2 G_PER_L Role: Carbon source; Properties: Organic compound, Defined component, Simple component
7 Biotin
C10H16N2O3S 244.316 g/mol
4 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
8 p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
10 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
9 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
10 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
10 Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
20 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
11 Folic acid
C19H19N7O6 441.404 g/mol
4 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 Vitamin B12 0.2 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 Riboflavin
C17H20N4O6 376.369 g/mol
10 G_PER_L CHEBI:17015
riboflavin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Nicotinamide
C6H6N2O 122.127 g/mol
10 G_PER_L CHEBI:17154
nicotinamide
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
15 Thiamine・HCl・2H2O 10 G_PER_L Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

Solution 1

Dose: 1030 G_PER_L

See mediadive_6108_Solution_1.yaml for composition

Solution 2

Dose: 0.2 G_PER_L

See mediadive_6109_Solution_2.yaml for composition

Modified Hutner's basal salts (see Medium [M941])

Dose: 20 G_PER_L

Cross-reference to Medium M941

Vitamin solution No. 6 (see below)

Dose: 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.826347Z togo-import Imported from TOGO Medium Source: JCM, ID: M1049
2026-01-28T19:48:25.194180Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:41.669192+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:09:11.673764Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:49.715661Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007565
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.065825+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M30" to "m30" for consistent matching
2026-05-16T09:03:07.186630+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:30.010036+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:29.798231+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).