Overview
- External ontology
- TOGO:M1065 — Modified Halobacteria Medium-3
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1065 Original source: JCM - JCM_M1008 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1008 Ingredient amounts use the exact formulation and preparation stage. Separate preparation excerpt: "Add components to distilled water and bring volume to 1.0 L.". Conditional solid option: "For preparation of solid medium, add 20.0 g/L agar.". Agar is included only in M372/M960 in this reviewed variant cohort; no step ordering is invented. JCM1008 explicitly states the modified 270 g/L NaCl and 15 g/L magnesium sulfate heptahydrate formulation, corroborated by TOGO M1065's g/L rows. The word supplemented leaves an operational ambiguity: adding magnesium to the unchanged 20 g/L base cannot lower its concentration to 15 g/L. No 35 g/L magnesium or 470 g/L sodium chloride sum is inferred, and this curation does not resolve that preparation ambiguity.
Composition
flowchart LR
CultureMech_007582["modified_halobacteria_medium_3"]
ing0_distilled_water["distilled water"]
CultureMech_007582 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007582 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007582 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_007582 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007582 --> ing4_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_4H2O -.-> CHEBI_86368
ing5_Sodium_glutamate["Sodium glutamate"]
CultureMech_007582 --> ing5_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing5_Sodium_glutamate -.-> CHEBI_64220
ing6_Trisodium_citrate["Trisodium citrate"]
CultureMech_007582 --> ing6_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate -.-> CHEBI_53258
ing7_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_007582 --> ing7_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_4H2O -.-> CHEBI_86249
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007582 --> ing8_Yeast_extract__BD_Difco_
ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_007582 --> ing9_Casamino_acids__BD_Difco_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component Water makes the preparation up to 1.0 L. Its separate input volume and mass are unspecified; neither 1 g/L nor a fixed 1 L water dose is supported. | |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
15 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component Use the recipe-specific final formulation, not the copied base amount. JCM1008 explicitly states the modified 270 g/L NaCl and 15 g/L magnesium sulfate heptahydrate formulation, corroborated by TOGO M1065's g/L rows. The word supplemented leaves an operational ambiguity: adding magnesium to the unchanged 20 g/L base cannot lower its concentration to 15 g/L. No 35 g/L magnesium or 470 g/L sodium chloride sum is inferred, and this curation does not resolve that preparation ambiguity. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
270 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound Use the recipe-specific final formulation, not the copied base amount. JCM1008 explicitly states the modified 270 g/L NaCl and 15 g/L magnesium sulfate heptahydrate formulation, corroborated by TOGO M1065's g/L rows. The word supplemented leaves an operational ambiguity: adding magnesium to the unchanged 20 g/L base cannot lower its concentration to 15 g/L. No 35 g/L magnesium or 470 g/L sodium chloride sum is inferred, and this curation does not resolve that preparation ambiguity. |
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component 2.0 g in the source preparation brought to 1.0 L gives 2 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". |
| 5 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 MG_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component 0.36 mg in the source preparation brought to 1.0 L gives 0.36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". |
| 6 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component 1.0 g in the source preparation brought to 1.0 L gives 1 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". |
| 7 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component 3.0 g in the source preparation brought to 1.0 L gives 3 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". |
| 8 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 MG_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component 36.0 mg in the source preparation brought to 1.0 L gives 36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". |
| 9 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". | |
| 10 | Casamino acids (BD-Difco) | 5 G_PER_L | Properties: Undefined component 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.857192Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1065 |
| 2026-01-28T19:48:39.841252Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:49.842335Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007582 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.194343+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Halobacteria Medium-3" to "modified_halobacteria_medium_3" for consistent matching |
| 2026-06-05T07:40:30.134609+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:21.004181+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-10-09T14:23:51.976655+00:00 | codex | CURATED_HALOBACTERIA_VARIANT_CONCENTRATIONS_AND_PREPARATION_STAGES | Source-specific ingredient and variant review. Every change has an exact PURL-backed excerpt. Prepared-base quantities, unquantified water/titrant, source limitations, chemical identities and prior history are preserved; no taxa-media assertions are added. |