Overview
- External ontology
- TOGO:M1067 — Aminobacterium Shuijingfangii Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1067 Original source: JCM - JCM_M1010 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1010
Composition
flowchart LR
CultureMech_007584["aminobacterium_shuijingfangii_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007584 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007584 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KH2PO4["KH2PO4"]
CultureMech_007584 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_007584 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2HPO4["K2HPO4"]
CultureMech_007584 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KCl["KCl"]
CultureMech_007584 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007584 --> ing6_Yeast_extract__BD_Difco_
ing7_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007584 --> ing7_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007584 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Nitrogen_gas["Nitrogen gas"]
CultureMech_007584 --> ing9_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_Nitrogen_gas -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 8 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1.38 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
1% CaCl2・2H2O solution
Dose: 15 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
2% MgCl2・6H2O solution
Dose: 20 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
1% Resazurin solution
Dose: 1 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Trace element solution SL--10 (see Medium [M433])
Dose: 1 G_PER_L
Cross-reference to Medium M433
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
10% (w/v) NaHCO3 solution*
Dose: 0.25 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
3% (w/v) Na2S・9H2O solution
Dose: 0.1 G_PER_L
Role: Reducing agent; Properties: Inorganic compound, Solution, Defined component
0.5 M L--Serine solution
Dose: 0.1 G_PER_L
Role: Nitrogen source; Properties: Defined component, Solution, Organic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.860826Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1067 |
| 2026-01-28T19:48:40.775516Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:05:08.650704+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T06:19:49.860120Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007584 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.212039+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Aminobacterium Shuijingfangii Medium" to "aminobacterium_shuijingfangii_medium" for consistent matching |
| 2026-05-16T09:03:07.318119+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.155261+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.831644+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |