Overview
- External ontology
- TOGO:M1070 — Acidithrix Ferrooydans Medium
- Applications
-
- Microbial cultivation
- pH
- 2.5
- Notes
- TOGO M1070 records JCM Medium 1012 with 100 ml/L UBS solution from M752/JCM 729, 10 ml/L Trace minerals from M142/JCM 151, 10 ml/L Ni-Se-W solution from M236/JCM 244, glucose, yeast extract, and distilled water. JCM 1012 adjusts the base to pH 2.5 with H2SO4, autoclaves it, then adds filter-sterilized 1 M FeSO4 solution at pH 2.0 after cooling.
Composition
flowchart LR
CultureMech_007588["acidithrix_ferrooydans_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007588 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007588 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_007588 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_H2SO4["H2SO4"]
CultureMech_007588 --> ing3_H2SO4
CHEBI_26836["CHEBI:26836"]
ing3_H2SO4 -.-> CHEBI_26836
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M1070 / JCM Medium 1012 lists 1.0 L Distilled water. |
| 2 | Yeast extract | 0.2 G_PER_L | TOGO M1070 / JCM Medium 1012 lists 0.2 g/L Yeast extract. | |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.9 G_PER_L |
CHEBI:17234
glucose
|
TOGO M1070 / JCM Medium 1012 lists 0.9 g/L Glucose. |
| 4 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
TOGO M1070 / JCM Medium 1012 adjusts the medium to pH 2.5 with H2SO4. |
Preparation
- Mix UBS solution, Trace minerals, Ni-Se-W solution, glucose, yeast extract, and distilled water thoroughly.
- Adjust the medium to pH 2.5 with H2SO4.
- Autoclave the acidified base medium.
- Filter-sterilize 1 M FeSO4 solution at pH 2.0.
- After cooling, add 5.0 ml/L filter-sterilized 1 M FeSO4 solution at pH 2.0.
Solutions
UBS solution
Dose: 100.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2SO4 | 14.0 G_PER_L | TOGO M752 / JCM Medium 729 lists 14.0 g/L Na2SO4. |
| (NH4)2SO4 | 30.0 G_PER_L | TOGO M752 / JCM Medium 729 lists 30.0 g/L (NH4)2SO4. |
| KCl | 1.0 G_PER_L | TOGO M752 / JCM Medium 729 lists 1.0 g/L KCl. |
| MgSO4 x 7H2O | 5.0 G_PER_L | TOGO M752 / JCM Medium 729 lists 5.0 g/L MgSO4 x 7H2O. |
| K2HPO4 | 0.5 G_PER_L | TOGO M752 / JCM Medium 729 lists 0.5 g/L K2HPO4. |
| Ca(NO3)2 x 4H2O | 0.14 G_PER_L | TOGO M752 / JCM Medium 729 lists 0.14 g/L Ca(NO3)2 x 4H2O. |
| Distilled water | 1.0 L | TOGO M752 / JCM Medium 729 lists 1.0 L Distilled water. |
TOGO M1070 / JCM Medium 1012 adds 100.0 ml/L UBS solution from M752/JCM 729.
Trace minerals
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | TOGO M142 / JCM Medium 151 lists 1.5 g/L Nitrilotriacetic acid. |
| MgSO4 x 7H2O | 3.0 G_PER_L | TOGO M142 / JCM Medium 151 lists 3.0 g/L MgSO4 x 7H2O. |
| MnSO4 x n H2O | 0.5 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.5 g/L MnSO4 x n H2O. |
| NaCl | 1.0 G_PER_L | TOGO M142 / JCM Medium 151 lists 1.0 g/L NaCl. |
| FeSO4 x 7H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L FeSO4 x 7H2O. |
| CoSO4 x 7H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L CoSO4 x 7H2O. |
| CaCl2 x 2H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L CaCl2 x 2H2O. |
| ZnSO4 x 7H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L ZnSO4 x 7H2O. |
| CuSO4 x 5H2O | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L CuSO4 x 5H2O. |
| AlK(SO4)2 | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L AlK(SO4)2. |
| H3BO3 | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L H3BO3. |
| Na2MoO4 x 2H2O | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L Na2MoO4 x 2H2O. |
| Distilled water | 1.0 L | TOGO M142 / JCM Medium 151 lists 1.0 L Distilled water. |
| KOH solution | variable VARIABLE | TOGO M142 / JCM Medium 151 adjusts nitrilotriacetic acid to pH 6.5 with KOH solution before adding minerals. |
TOGO M1070 / JCM Medium 1012 adds 10.0 ml/L Trace minerals from M142/JCM 151.
Ni-Se-W solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NiCl2 x 6H2O | 25.0 MG_PER_L | TOGO M236 / JCM Medium 244 lists 25.0 mg/L NiCl2 x 6H2O. |
| (NH4)2Ni(SO4)2 x 6H2O | 2.0 G_PER_L | TOGO M236 / JCM Medium 244 lists 2.0 g/L (NH4)2Ni(SO4)2 x 6H2O. |
| Na2SeO3 | 0.3 MG_PER_L | TOGO M236 / JCM Medium 244 lists 0.3 mg/L Na2SeO3. |
| Na2SeO4 | 10.0 MG_PER_L | TOGO M236 / JCM Medium 244 lists 10.0 mg/L Na2SeO4. |
| Na2WO4 x 2H2O | 10.0 MG_PER_L | TOGO M236 / JCM Medium 244 lists 10.0 mg/L Na2WO4 x 2H2O. |
| Distilled water | 1.0 L | TOGO M236 / JCM Medium 244 lists 1.0 L Distilled water. |
TOGO M1070 / JCM Medium 1012 adds 10.0 ml/L Ni-Se-W solution from M236/JCM 244.
1 M FeSO4 solution (pH 2.0)
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeSO4 | variable VARIABLE | TOGO M1070 / JCM Medium 1012 lists a 1 M FeSO4 solution at pH 2.0; the molar stock concentration is preserved in this note. |
TOGO M1070 / JCM Medium 1012 adds 5.0 ml/L filter-sterilized 1 M FeSO4 solution at pH 2.0 after cooling.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.866811Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1070 |
| 2026-01-28T19:48:36.267079Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:05:00.467968+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:49.883285Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007588 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.236378+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidithrix Ferrooydans Medium" to "acidithrix_ferrooydans_medium" for consistent matching |
| 2026-05-16T09:03:07.342111+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.183956+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1070_score15.py | RESOLVED_TOGO_M1070_SCORE15 | TOGO M1070 records JCM Medium 1012 with 100 ml/L UBS solution from M752/JCM 729, 10 ml/L Trace minerals from M142/JCM 151, 10 ml/L Ni-Se-W solution from M236/JCM 244, glucose, yeast extract, and distilled water. JCM 1012 adjusts the base to pH 2.5 with H2SO4, autoclaves it, then adds filter-sterilized 1 M FeSO4 solution at pH 2.0 after cooling. |