acetivibrio_medium

CultureMech:007589 bacterial COMPLEX LIQUID
Original name: Acetivibrio Medium

Overview

External ontology
TOGO:M1071 — Acetivibrio Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1071 Original source: JCM - JCM_M1013 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1013

Composition

flowchart LR
    CultureMech_007589["acetivibrio_medium"]
    ing0_NaHCO3__5___w_v_["NaHCO3 (5%, w/v)"]
    CultureMech_007589 --> ing0_NaHCO3__5___w_v_
    ing1_Sucrose__20___w_v_["Sucrose (20%, w/v)"]
    CultureMech_007589 --> ing1_Sucrose__20___w_v_
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007589 --> ing2_Yeast_extract
    ing3_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_007589 --> ing3_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_9H2O -.-> CHEBI_76209
    ing4_Basal_medium__see_below_["Basal medium (see below)"]
    CultureMech_007589 --> ing4_Basal_medium__see_below_
    ing5_N2["N2"]
    CultureMech_007589 --> ing5_N2
    CHEBI_17997["CHEBI:17997"]
    ing5_N2 -.-> CHEBI_17997
    ing6_Distilled_water["Distilled water"]
    CultureMech_007589 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007589 --> ing7_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_2H2O -.-> CHEBI_86158
    ing8_KH2PO4["KH2PO4"]
    CultureMech_007589 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_NH4Cl["NH4Cl"]
    CultureMech_007589 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_Resazurin["Resazurin"]
    CultureMech_007589 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007589 --> ing11_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing11_MgCl2_6H2O -.-> CHEBI_86345

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 NaHCO3 (5%, w/v) 30 G_PER_L Role: Buffer; Properties: Defined component, Inorganic compound, Solution
2 Sucrose (20%, w/v) 10 G_PER_L Role: Carbon source
3 Yeast extract 50 G_PER_L Role: Nutrient source; Properties: Undefined component
4 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.25 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound
5 Basal medium (see below) 950 G_PER_L
6 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
7 Distilled water
H2O 18.015 g/mol
950 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
8 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 KH2PO4
H2O4P.K 136.084 g/mol
1.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
10 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
11 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
12 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound

Solutions

Trace element solution SL--10 (see Medium [M433])

Dose: 1 G_PER_L

Cross-reference to Medium M433

Selenite--tungstate solution (see Medium [M558])

Dose: 1 G_PER_L

Cross-reference to Medium M558

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.867995Z togo-import Imported from TOGO Medium Source: JCM, ID: M1071
2026-01-28T19:48:33.231377Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:55.057993+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:49.890241Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007589
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.243366+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Acetivibrio Medium" to "acetivibrio_medium" for consistent matching
2026-05-16T09:03:07.350030+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.192323+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.842779+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.