Overview
- External ontology
- TOGO:M1081 — Desulfocarbo Indianensis Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1081 Original source: JCM - JCM_M1020 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1020
Composition
flowchart LR
CultureMech_007597["desulfocarbo_indianensis_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007597 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007597 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007597 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_007597 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_007597 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_007597 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007597 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_007597 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_007597 --> ing8_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_6H2O -.-> CHEBI_34887
ing9_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_007597 --> ing9_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing9_Na2SeO3_5H2O -.-> CHEBI_131361
ing10_Na2SO4["Na2SO4"]
CultureMech_007597 --> ing10_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing10_Na2SO4 -.-> CHEBI_32149
ing11_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_007597 --> ing11_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing11_Na2WO4_2H2O -.-> CHEBI_63939
ing12_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_007597 --> ing12_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing12_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing13_Sodium_benzoate["Sodium benzoate"]
CultureMech_007597 --> ing13_Sodium_benzoate
CHEBI_113455["CHEBI:113455"]
ing13_Sodium_benzoate -.-> CHEBI_113455
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007597 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_007597 --> ing15_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen_gas -.-> CHEBI_17997
ing16_5__Na2S_9H2O__autoclaved_["5% Na2S・9H2O (autoclaved)"]
CultureMech_007597 --> ing16_5__Na2S_9H2O__autoclaved_
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
8.8 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.34 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
9.3 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 10 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.26 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.084 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
2.7 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 14 |
Sodium benzoate
C7H5O2.Na 144.105 g/mol
|
0.36 G_PER_L |
CHEBI:113455
sodium benzoate
|
Properties: Defined component, Organic compound, Simple component |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 | 5% Na2S・9H2O (autoclaved) | 5 G_PER_L | Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound |
Solutions
Trace element solution (see Medium [M180])
Dose: 10 G_PER_L
Cross-reference to Medium M180
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
8% NaHCO3 solution (filter-sterilized)
Dose: 60 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.877226Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1081 |
| 2026-01-28T19:48:32.618930Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:54.164406+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:49.933660Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007597 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.288642+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfocarbo Indianensis Medium" to "desulfocarbo_indianensis_medium" for consistent matching |
| 2026-05-16T09:03:07.391320+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.239025+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.851991+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |