Overview
- External ontology
- TOGO:M1094 — Dissulfurimicrobium Hydrothermale Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1094 Original source: JCM - JCM_M1030 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1030
Composition
flowchart LR
CultureMech_007611["dissulfurimicrobium_hydrothermale_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007611 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_007611 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_007611 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_007611 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_007611 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_007611 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Sulfur__powder_["Sulfur (powder)"]
CultureMech_007611 --> ing6_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing6_Sulfur__powder_ -.-> CHEBI_33403
ing7_Ferrihydrite_suspension__see_below_["Ferrihydrite suspension (see below)"]
CultureMech_007611 --> ing7_Ferrihydrite_suspension__see_below_
ing8_CO2["CO2"]
CultureMech_007611 --> ing8_CO2
CHEBI_16526["CHEBI:16526"]
ing8_CO2 -.-> CHEBI_16526
ing9_NaHCO3["NaHCO3"]
CultureMech_007611 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Ferrihydrite["Ferrihydrite"]
CultureMech_007611 --> ing10_Ferrihydrite
CHEBI_192761["CHEBI:192761"]
ing10_Ferrihydrite -.-> CHEBI_192761
ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_007611 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_007611 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_007611 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_007611 --> ing14_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2_6H2O -.-> CHEBI_35696
ing15_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_007611 --> ing15_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_6H2O -.-> CHEBI_34887
ing16_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_007611 --> ing16_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_7H2O -.-> CHEBI_32312
ing17_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_007611 --> ing17_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_2H2O -.-> CHEBI_86318
ing18_Na2SO4["Na2SO4"]
CultureMech_007611 --> ing18_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing18_Na2SO4 -.-> CHEBI_32149
ing19_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_007611 --> ing19_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_2H2O -.-> CHEBI_63939
ing20__NH4_2Fe_SO4_2_6H2O["(NH4)2Fe(SO4)2·6H2O"]
CultureMech_007611 --> ing20__NH4_2Fe_SO4_2_6H2O
ing21_KI["KI"]
CultureMech_007611 --> ing21_KI
CHEBI_8346["CHEBI:8346"]
ing21_KI -.-> CHEBI_8346
ing22_KBr["KBr"]
CultureMech_007611 --> ing22_KBr
CHEBI_32030["CHEBI:32030"]
ing22_KBr -.-> CHEBI_32030
ing23_Na2SiO3_9H2O["Na2SiO3·9H2O"]
CultureMech_007611 --> ing23_Na2SiO3_9H2O
CHEBI_132108["CHEBI:132108"]
ing23_Na2SiO3_9H2O -.-> CHEBI_132108
ing24_AlCl3_6H2O["AlCl3·6H2O"]
CultureMech_007611 --> ing24_AlCl3_6H2O
CHEBI_30115["CHEBI:30115"]
ing24_AlCl3_6H2O -.-> CHEBI_30115
ing25_BaCl2["BaCl2"]
CultureMech_007611 --> ing25_BaCl2
CHEBI_63317["CHEBI:63317"]
ing25_BaCl2 -.-> CHEBI_63317
ing26_Na2SeO4["Na2SeO4"]
CultureMech_007611 --> ing26_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing26_Na2SeO4 -.-> CHEBI_77775
ing27_SrSO4["SrSO4"]
CultureMech_007611 --> ing27_SrSO4
ing28_CrK_SO4_2_12H2O["CrK(SO4)2·12H2O"]
CultureMech_007611 --> ing28_CrK_SO4_2_12H2O
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1001.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 800.0, 200.0, 1.0] |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.33 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Sulfur (powder) | 5 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Ferrihydrite suspension (see below) | 200 G_PER_L | ||
| 9 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Ferrihydrite | variable VARIABLE |
CHEBI:192761
ferrihydrite
|
Role: Mineral source |
| 12 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.42 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.62 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
19.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
23.8 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
16.6 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
14.4 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.17 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2SO4
2Na.O4S 142.043 g/mol
|
71 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
5.49 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | (NH4)2Fe(SO4)2·6H2O | 78.4 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 22 |
KI
I.K 166.002 g/mol
|
16.6 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
KBr
Br.K 119.002 g/mol
|
11.9 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2SiO3·9H2O
9H2O.2Na.O3Si 284.198 g/mol
|
14.2 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
AlCl3·6H2O
AlCl3.6H2O 241.431 g/mol
|
2.41 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
BaCl2
Ba.2Cl 208.234 g/mol
|
2.08 G_PER_L |
CHEBI:63317
barium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
9.45 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 | SrSO4 | 0.92 G_PER_L | Role: Mineral source | |
| 29 | CrK(SO4)2·12H2O | 0.5 G_PER_L | Role: Mineral source |
Solutions
Trace elements solution (see below)
Dose: 10 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:53.902600Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1094 |
| 2026-01-28T19:48:13.996955Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: CO2; Added default concentration for ingredient: Ferrihydrite |
| 2026-03-09T08:04:23.203612+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:51.441695Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:50.047382Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007611 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.406447+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Dissulfurimicrobium Hydrothermale Medium" to "dissulfurimicrobium_hydrothermale_medium" for consistent matching |
| 2026-05-16T09:03:07.499637+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.354713+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:29.882586+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:46.885540+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |