Overview
- External ontology
- TOGO:M109 — R Agar With Catalase
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- TOGO M109 prepares R Agar from TOGO M19/JCM Medium 26 and supplements it with 60 mg/L filter-sterilized catalase.
Composition
flowchart LR
CultureMech_007617["r_agar_with_catalase"]
ing0_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_007617 --> ing0_Bacto_peptone__BD_Difco_
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007617 --> ing1_Yeast_extract__BD_Difco_
ing2_Malt_extract__BD_Difco_["Malt extract (BD-Difco)"]
CultureMech_007617 --> ing2_Malt_extract__BD_Difco_
ing3_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_007617 --> ing3_Casamino_acids__BD_Difco_
ing4_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
CultureMech_007617 --> ing4_Beef_extract__BD_Difco_
ing5_Glycerol["Glycerol"]
CultureMech_007617 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_Tween_80["Tween 80"]
CultureMech_007617 --> ing6_Tween_80
CHEBI_53426["CHEBI:53426"]
ing6_Tween_80 -.-> CHEBI_53426
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_007617 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Agar["Agar"]
CultureMech_007617 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Catalase__Sigma_C_10_["Catalase (Sigma C-10)"]
CultureMech_007617 --> ing9_Catalase__Sigma_C_10_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone (BD-Difco) | 10 G_PER_L | TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. | |
| 2 | Yeast extract (BD-Difco) | 5 G_PER_L | TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. | |
| 3 | Malt extract (BD-Difco) | 5 G_PER_L | TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. | |
| 4 | Casamino acids (BD-Difco) | 5 G_PER_L | TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. | |
| 5 | Beef extract (BD-Difco) | 2 G_PER_L | TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. | |
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. |
| 7 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.05 G_PER_L |
CHEBI:53426
polysorbate 80
|
TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. |
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
TOGO M19 and JCM Medium 26 list this component for R Agar; TOGO variants reuse the same agar base. |
| 10 | Catalase (Sigma C-10) | 0.06 G_PER_L | TOGO M109 lists 60 mg Catalase per liter of R Agar and separately sterilizes the catalase by filtration. |
Preparation
- Autoclave the TOGO M19 R Agar base.
- Sterilize catalase separately by filtration.
- Add 60 mg/L sterile catalase to the R Agar base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.894570Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M109 |
| 2026-03-09T08:04:43.355249+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:50.096532Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007617 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:08.457584+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R Agar With Catalase" to "r_agar_with_catalase" for consistent matching |
| 2026-05-16T09:03:07.556048+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_bacterial_togo_score40.py | RESOLVED_BACTERIAL_TOGO_SCORE40_GRAPH | TOGO M109 prepares R Agar from TOGO M19/JCM Medium 26 and supplements it with 60 mg/L filter-sterilized catalase. |