Overview
- External ontology
- TOGO:M1118 — Endomicrobium Proavitum RSA Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1118 Original source: JCM - JCM_M1051 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1051
Composition
flowchart LR
CultureMech_007638["endomicrobium_proavitum_rsa_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007638 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007638 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007638 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_007638 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_007638 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_007638 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007638 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_007638 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007638 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Nitrogen_gas["Nitrogen gas"]
CultureMech_007638 --> ing9_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_Nitrogen_gas -.-> CHEBI_17997
ing10_0_1_M_L__Cysteine_HCl_H2O["0.1 M L--Cysteine・HCl・H2O"]
CultureMech_007638 --> ing10_0_1_M_L__Cysteine_HCl_H2O
ing11_4__Hydroxyphenyl_acetic_acid["4--Hydroxyphenyl acetic acid"]
CultureMech_007638 --> ing11_4__Hydroxyphenyl_acetic_acid
CHEBI_18101["CHEBI:18101"]
ing11_4__Hydroxyphenyl_acetic_acid -.-> CHEBI_18101
ing12_Phenyl_propionic_acid["Phenyl propionic acid"]
CultureMech_007638 --> ing12_Phenyl_propionic_acid
CHEBI_28631["CHEBI:28631"]
ing12_Phenyl_propionic_acid -.-> CHEBI_28631
ing13_Phenyl_acetic_acid["Phenyl acetic acid"]
CultureMech_007638 --> ing13_Phenyl_acetic_acid
CHEBI_30745["CHEBI:30745"]
ing13_Phenyl_acetic_acid -.-> CHEBI_30745
ing14_3__Indolyl_acetic_acid["3--Indolyl acetic acid"]
CultureMech_007638 --> ing14_3__Indolyl_acetic_acid
CHEBI_16411["CHEBI:16411"]
ing14_3__Indolyl_acetic_acid -.-> CHEBI_16411
ing15_N2["N2"]
CultureMech_007638 --> ing15_N2
ing15_N2 -.-> CHEBI_17997
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
945 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.4 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 | 0.1 M L--Cysteine・HCl・H2O | 10 G_PER_L | Properties: Defined component, Solution, Organic compound | |
| 12 |
4--Hydroxyphenyl acetic acid
C8H8O3 152.149 g/mol
|
76 G_PER_L |
CHEBI:18101
4-hydroxyphenylacetic acid
|
Role: Carbon source |
| 13 |
Phenyl propionic acid
C9H10O2 150.177 g/mol
|
75 G_PER_L |
CHEBI:28631
3-phenylpropionic acid
|
Role: Carbon source |
| 14 |
Phenyl acetic acid
C8H8O2 136.15 g/mol
|
68 G_PER_L |
CHEBI:30745
phenylacetic acid
|
Role: Carbon source |
| 15 |
3--Indolyl acetic acid
C10H9NO2 175.187 g/mol
|
88 G_PER_L |
CHEBI:16411
indole-3-acetic acid
|
Role: Carbon source |
| 16 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
8% NaHCO3 solution*
Dose: 30 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
10% Yeast extract solution*
Dose: 2 G_PER_L
Role: Nutrient source; Properties: Undefined component
1 M Glucose solution*
Dose: 5 G_PER_L
Role: Carbon source; Properties: Solution, Defined component, Organic compound
10% Casamino acids solution*
Dose: 2 G_PER_L
Properties: Solution, Undefined component
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
Trace vitamins solution (see Medium [M278])
Dose: 1 G_PER_L
Cross-reference to Medium M278
Fatty acid mixture (see Medium [M258])
Dose: 1 G_PER_L
Cross-reference to Medium M258
Aromatic fatty acids solution (see below)
Dose: 1 G_PER_L
250 mM Dithiothreitol solution
Dose: 4 G_PER_L
Properties: Solution, Defined component, Organic compound
20 mM NaOH solution
Dose: 100 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:53.953055Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1118 |
| 2026-01-28T19:48:17.667982Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:29.262852+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 12 solution(s) from ingredients |
| 2026-03-13T06:19:50.256679Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007638 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.622724+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Endomicrobium Proavitum RSA Medium" to "endomicrobium_proavitum_rsa_medium" for consistent matching |
| 2026-05-16T09:03:07.694909+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.566571+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.954841+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |