573c_medium

CultureMech:007639 bacterial COMPLEX LIQUID
Original name: 573C Medium

Overview

External ontology
TOGO:M1119 — 573C Medium
Applications
  • Microbial cultivation
pH range
{'min': 4.8, 'max': 5.0}
Notes
TOGO M1119 records JCM Medium 1052 with carboxymethyl cellulose sodium salt, ammonium sulfate, KH2PO4, glucose, yeast extract, tryptone, 25% MgSO4 x 7H2O solution, 7% CaCl2 x 2H2O solution, 2% FeCl3 x 6H2O solution, and distilled water. JCM 1052 adjusts the medium to pH 4.8-5.0, autoclaves those stock solutions separately, and adds all solutions aseptically to the medium.

Composition

flowchart LR
    CultureMech_007639["573c_medium"]
    ing0_Carboxymethyl_cellulose__sodium_salt["Carboxymethyl cellulose, sodium salt"]
    CultureMech_007639 --> ing0_Carboxymethyl_cellulose__sodium_salt
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007639 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007639 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Glucose["Glucose"]
    CultureMech_007639 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007639 --> ing4_Yeast_extract__BD_Difco_
    ing5_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007639 --> ing5_Tryptone__BD_Difco_
    ing6_Distilled_water["Distilled water"]
    CultureMech_007639 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Carboxymethyl cellulose, sodium salt 2.5 G_PER_L JCM Medium 1052 lists 2.5 g/L Carboxymethyl cellulose, sodium salt.
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
 JCM Medium 1052 lists 1.3 g/L (NH4)2SO4.
3 KH2PO4
H2O4P.K 136.084 g/mol
0.37 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 JCM Medium 1052 lists 0.37 g/L KH2PO4.
4 Glucose
C6H12O6 180.156 g/mol
1.0 G_PER_L CHEBI:17234
glucose
 JCM Medium 1052 lists 1.0 g/L Glucose.
5 Yeast extract (BD-Difco) 0.25 G_PER_L JCM Medium 1052 lists 0.25 g/L Yeast extract (BD-Difco).
6 Tryptone (BD-Difco) 0.9 G_PER_L JCM Medium 1052 lists 0.9 g/L Tryptone (BD-Difco).
7 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 1052 lists 1.0 L Distilled water.

Preparation

  1. Mix carboxymethyl cellulose sodium salt, ammonium sulfate, KH2PO4, glucose, yeast extract, tryptone, and distilled water.
  2. Adjust to pH 4.8-5.0.
  3. Autoclave the base medium and the MgSO4 x 7H2O, CaCl2 x 2H2O, and FeCl3 x 6H2O stock solutions separately at 121 degrees C for 15 min.
  4. Aseptically add all stock solutions to the medium.

Solutions

25% MgSO4 x 7H2O solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgSO4 x 7H2O 25.0 PERCENT_W_V 25% MgSO4 x 7H2O solution is represented from the stock label as 25.0 % w/v MgSO4 x 7H2O.
JCM Medium 1052 lists 1.0 ml/L 25% MgSO4 x 7H2O solution, autoclaved separately and added aseptically to the medium.

7% CaCl2 x 2H2O solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
CaCl2 x 2H2O 7.0 PERCENT_W_V 7% CaCl2 x 2H2O solution is represented from the stock label as 7.0 % w/v CaCl2 x 2H2O.
JCM Medium 1052 lists 1.0 ml/L 7% CaCl2 x 2H2O solution, autoclaved separately and added aseptically to the medium.

2% FeCl3 x 6H2O solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl3 x 6H2O 2.0 PERCENT_W_V 2% FeCl3 x 6H2O solution is represented from the stock label as 2.0 % w/v FeCl3 x 6H2O.
JCM Medium 1052 lists 1.0 ml/L 2% FeCl3 x 6H2O solution, autoclaved separately and added aseptically to the medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.955952Z togo-import Imported from TOGO Medium Source: JCM, ID: M1119
2026-01-28T19:48:39.476968Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:06.379621+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:50.264988Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007639
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.631874+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "573C Medium" to "573c_medium" for consistent matching
2026-05-16T09:03:07.705360+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:30.576707+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.961285+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00 repair_togo_m1119_score15.py RESOLVED_TOGO_M1119_SCORE15 TOGO M1119 records JCM Medium 1052 with carboxymethyl cellulose sodium salt, ammonium sulfate, KH2PO4, glucose, yeast extract, tryptone, 25% MgSO4 x 7H2O solution, 7% CaCl2 x 2H2O solution, 2% FeCl3 x 6H2O solution, and distilled water. JCM 1052 adjusts the medium to pH 4.8-5.0, autoclaves those stock solutions separately, and adds all solutions aseptically to the medium.