pygv_marine_medium_b

Overview

External ontology

TOGO:M1126 — PYGV Marine Medium (B)

Applications

• Microbial cultivation

pH range

{'min': 7.2, 'max': 7.4}

Notes

TOGO M1126 records the liquid JCM Medium 1059 PYGV Marine Medium (B) formulation with Bacto peptone, yeast extract, mineral salt solution from JCM Medium 304, 2.5% glucose solution, vitamin solution from JCM Medium 304, artificial seawater from JCM Medium 304, distilled water, and KOH for optional pH adjustment. JCM 1059 gently heats the base, autoclaves it, cools it to 45-50 degrees C, adds filter-sterilized glucose and vitamin solutions aseptically, and adjusts pH to 7.2-7.4 with sterile KOH if necessary.

Composition

flowchart LR
    CultureMech_007647["pygv_marine_medium_b"]
    ing0_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_007647 --> ing0_Bacto_peptone__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007647 --> ing1_Yeast_extract__BD_Difco_
    ing2_Distilled_water["Distilled water"]
    CultureMech_007647 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_KOH["KOH"]
    CultureMech_007647 --> ing3_KOH
    CHEBI_32035["CHEBI:32035"]
    ing3_KOH -.-> CHEBI_32035

Ingredients (4)

Preparation

1. Mix peptone, yeast extract, mineral salt solution, artificial seawater, and distilled water.
2. Gently heat and bring to a boil.
3. Autoclave at 121 degrees C for 15 min.
4. Cool to 45-50 degrees C.
5. Aseptically add the filter-sterilized glucose solution and vitamin solution.
6. Adjust to pH 7.2-7.4 with sterile KOH, if necessary.

Solutions

Target organisms

No target organisms are recorded.

Provenance