pygv_marine_medium_b

Overview

External ontology

TOGO:M1127 — PYGV Marine Medium (B)

Applications

• Microbial cultivation

pH range

{'min': 7.2, 'max': 7.4}

Notes

TOGO M1127 records JCM Medium 1059 with Bacto peptone, yeast extract, mineral salt solution from JCM Medium 304, 2.5% glucose solution, vitamin solution from JCM Medium 304, artificial seawater from JCM Medium 304, distilled water, KOH for optional pH adjustment, and 15 g/L agar for the solid formulation. JCM 1059 gently heats the base, autoclaves it, cools it to 45-50 degrees C, adds filter-sterilized glucose and vitamin solutions aseptically, and adjusts pH to 7.2-7.4 with sterile KOH if necessary.

Composition

flowchart LR
    CultureMech_007648["pygv_marine_medium_b"]
    ing0_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_007648 --> ing0_Bacto_peptone__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007648 --> ing1_Yeast_extract__BD_Difco_
    ing2_Agar["Agar"]
    CultureMech_007648 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_007648 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_KOH["KOH"]
    CultureMech_007648 --> ing4_KOH
    CHEBI_32035["CHEBI:32035"]
    ing4_KOH -.-> CHEBI_32035

Ingredients (5)

Preparation

1. Mix peptone, yeast extract, mineral salt solution, artificial seawater, agar, and distilled water.
2. Gently heat and bring to a boil.
3. Autoclave at 121 degrees C for 15 min.
4. Cool to 45-50 degrees C.
5. Aseptically add the filter-sterilized glucose solution and vitamin solution.
6. Adjust to pH 7.2-7.4 with sterile KOH, if necessary.

Solutions

Target organisms

No target organisms are recorded.

Provenance