halobacteria_mh4_2_medium

CultureMech:007652 archaea COMPLEX SOLID_AGAR
Original name: Halobacteria MH4.2 Medium

Overview

External ontology
TOGO:M1130 — Halobacteria MH4.2 Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1130 Original source: JCM - JCM_M1061-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1061

Composition

flowchart LR
    CultureMech_007652["halobacteria_mh4_2_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007652 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007652 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007652 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007652 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2HPO4["K2HPO4"]
    CultureMech_007652 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_007652 --> ing5_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_4H2O -.-> CHEBI_86368
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007652 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144
    ing7_K2SO4["K2SO4"]
    CultureMech_007652 --> ing7_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing7_K2SO4 -.-> CHEBI_32036
    ing8_FeCl2_4H2O["FeCl2・4H2O"]
    CultureMech_007652 --> ing8_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_4H2O -.-> CHEBI_86249
    ing9_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
    CultureMech_007652 --> ing9_Trisodium_citrate_2H2O
    CHEBI_53258["CHEBI:53258"]
    ing9_Trisodium_citrate_2H2O -.-> CHEBI_53258
    ing10_agar["agar"]
    CultureMech_007652 --> ing10_agar
    CHEBI_2509["CHEBI:2509"]
    ing10_agar -.-> CHEBI_2509
    ing11_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007652 --> ing11_Yeast_extract__BD_Difco_
    ing12_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007652 --> ing12_Tryptone__BD_Difco_

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 NH4Cl
Cl.H4N 53.492 g/mol
0.6 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
5 K2HPO4
HO4P.2K 174.174 g/mol
0.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
6 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Simple component, Defined component, Organic compound
8 K2SO4
2K.O4S 174.259 g/mol
5 G_PER_L CHEBI:32036
potassium sulfate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
36 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
10 Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
1 G_PER_L CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Simple component, Organic compound, Defined component
11 agar 20 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
12 Yeast extract (BD-Difco) 2 G_PER_L Properties: Undefined component
13 Tryptone (BD-Difco) 2 G_PER_L Properties: Undefined component

Solutions

0.5 M Tris--HCl solution (pH 8.0)

Dose: 100 G_PER_L

Role: Buffer; Properties: Complex component, Defined component, Solution

SL-6 trace element solution (see Medium [M158])

Dose: 1 G_PER_L

Cross-reference to Medium M158

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.976143Z togo-import Imported from TOGO Medium Source: JCM, ID: M1130
2026-01-28T19:48:41.225206Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:09.433702+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:50.347653Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007652
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.718381+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halobacteria MH4.2 Medium" to "halobacteria_mh4_2_medium" for consistent matching
2026-05-16T09:03:07.768367+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:30.643535+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.382169+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:46.980934+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.