m25y_medium

CultureMech:007657 bacterial COMPLEX LIQUID
Original name: M25Y Medium

Overview

External ontology
TOGO:M1135 — M25Y Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1135 Original source: JCM - JCM_M1066 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1066

Composition

flowchart LR
    CultureMech_007657["m25y_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007657 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007657 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007657 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_007657 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007657 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_007657 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_K2HPO4["K2HPO4"]
    CultureMech_007657 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Resazurin["Resazurin"]
    CultureMech_007657 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007657 --> ing8_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_6H2O -.-> CHEBI_86345
    ing9_KCl["KCl"]
    CultureMech_007657 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_MES["MES"]
    CultureMech_007657 --> ing10_MES
    CHEBI_39010["CHEBI:39010"]
    ing10_MES -.-> CHEBI_39010
    ing11_0_2__Fe_NH4_2_SO4__6H2O__in_0_037__HCl_["0.2% Fe(NH4)2(SO4)・6H2O (in 0.037% HCl)"]
    CultureMech_007657 --> ing11_0_2__Fe_NH4_2_SO4__6H2O__in_0_037__HCl_
    ing12_HCl["HCl"]
    CultureMech_007657 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_N2["N2"]
    CultureMech_007657 --> ing13_N2
    CHEBI_17997["CHEBI:17997"]
    ing13_N2 -.-> CHEBI_17997

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
2.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 0.2 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.14 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
7 K2HPO4
HO4P.2K 174.174 g/mol
0.14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
8 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
9 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
4.3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 KCl
Cl.K 74.551 g/mol
0.34 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 MES 3.9 G_PER_L CHEBI:39010
MES
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
12 0.2% Fe(NH4)2(SO4)・6H2O (in 0.037% HCl) 1 G_PER_L Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
13 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
 Role: Solvating media; Properties: Defined component, Inorganic compound, Solution
14 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Trace elements (see Medium [M631])

Dose: 1 G_PER_L

Cross-reference to Medium M631

5% Na2S・9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.985701Z togo-import Imported from TOGO Medium Source: JCM, ID: M1135
2026-01-28T19:48:06.852537Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: HCl; Added default concentration for ingredient: N2
2026-03-09T08:04:11.038859+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:50.390152Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007657
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.762339+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M25Y Medium" to "m25y_medium" for consistent matching
2026-05-16T09:03:07.810611+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.687832+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.411574+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:46.998203+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.