saltwater_iron_oxidizing_bacteria_medium_ii

CultureMech:007659 bacterial DEFINED LIQUID
Original name: Saltwater Iron-Oxidizing Bacteria Medium-II

Overview

External ontology
TOGO:M1137 — Saltwater Iron-Oxidizing Bacteria Medium-II
Applications
  • Microbial cultivation
Notes
TOGO M1137 records JCM Medium 1068 as a saltwater iron-oxidizing bacteria medium. JCM 1068 mixes Artificial saltwater from JCM Medium 629 with Wolfe's mineral solution from JCM Medium 265, autoclaves the sealed vessels, aseptically adds filter-sterilized Trace vitamins from JCM Medium 197, 8% NaHCO3 solution, and freshly prepared 0.2 M FeCl2 solution, then replaces the gas phase with N2-CO2-O2 (80:20:3, v/v/v) and pressurizes it to 200 kPa.

Composition

flowchart LR
    CultureMech_007659["saltwater_iron_oxidizing_bacteria_medium_ii"]
    ing0_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007659 --> ing0_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing0_Carbon_dioxide_gas -.-> CHEBI_16526
    ing1_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007659 --> ing1_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing1_Nitrogen_gas -.-> CHEBI_17997
    ing2_Oxygen_gas["Oxygen gas"]
    CultureMech_007659 --> ing2_Oxygen_gas
    CHEBI_15379["CHEBI:15379"]
    ing2_Oxygen_gas -.-> CHEBI_15379

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 JCM Medium 1068 replaces the gas phase with N2-CO2-O2 (80:20:3, v/v/v) and pressurizes it to 200 kPa.
2 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 JCM Medium 1068 replaces the gas phase with N2-CO2-O2 (80:20:3, v/v/v) and pressurizes it to 200 kPa.
3 Oxygen gas
O2 31.998 g/mol
variable VARIABLE CHEBI:15379
dioxygen
 JCM Medium 1068 replaces the gas phase with N2-CO2-O2 (80:20:3, v/v/v) and pressurizes it to 200 kPa.

Preparation

  1. Mix Artificial saltwater and Wolfe's mineral solution, distribute the medium into culture vessels, and seal with butyl rubber stoppers.
  2. Autoclave at 121 degrees C for 15 min.
  3. Aseptically add filter-sterilized Trace vitamins, 8% NaHCO3 solution, and freshly prepared 0.2 M FeCl2 solution per liter.
  4. Replace the gas phase with an N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture.

Solutions

Artificial saltwater

Dose: 1000.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaCl 27.5 G_PER_L TOGO M642 / JCM Medium 629 lists 27.5 g/L NaCl.
MgCl2 x 6H2O 5.38 G_PER_L TOGO M642 / JCM Medium 629 lists 5.38 g/L MgCl2 x 6H2O.
MgSO4 x 7H2O 6.78 G_PER_L TOGO M642 / JCM Medium 629 lists 6.78 g/L MgSO4 x 7H2O.
KCl 0.72 G_PER_L TOGO M642 / JCM Medium 629 lists 0.72 g/L KCl.
NaHCO3 0.2 G_PER_L TOGO M642 / JCM Medium 629 lists 0.2 g/L NaHCO3.
CaCl2 x 2H2O 1.4 G_PER_L TOGO M642 / JCM Medium 629 lists 1.4 g/L CaCl2 x 2H2O.
NH4Cl 1.0 G_PER_L TOGO M642 / JCM Medium 629 lists 1.0 g/L NH4Cl.
KH2PO4 0.05 G_PER_L TOGO M642 / JCM Medium 629 lists 0.05 g/L KH2PO4.
Distilled water 1.0 L TOGO M642 / JCM Medium 629 lists 1.0 L Distilled water.
JCM Medium 1068 lists 1.0 L Artificial saltwater.

Wolfe's mineral solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Nitrilotriacetic acid 1.5 G_PER_L JCM Medium 151 lists 1.5 g/L Nitrilotriacetic acid.
MgSO4 x 7H2O 3.0 G_PER_L JCM Medium 151 lists 3.0 g/L MgSO4 x 7H2O.
MnSO4 x xH2O 0.5 G_PER_L JCM Medium 151 lists 0.5 g/L MnSO4 x xH2O.
NaCl 1.0 G_PER_L JCM Medium 151 lists 1.0 g/L NaCl.
FeSO4 x 7H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L FeSO4 x 7H2O.
CoSO4 x 7H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L CoSO4 x 7H2O.
CaCl2 x 2H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L CaCl2 x 2H2O.
ZnSO4 x 7H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L ZnSO4 x 7H2O.
CuSO4 x 5H2O 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L CuSO4 x 5H2O.
AlK(SO4)2 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L AlK(SO4)2.
H3BO3 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L H3BO3.
Na2MoO4 x 2H2O 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L Na2MoO4 x 2H2O.
Distilled water 1.0 L JCM Medium 151 lists 1.0 L Distilled water.
NiCl2 x 6H2O 0.02 G_PER_L TOGO M257 / JCM Medium 265 lists 0.02 g/L NiCl2 x 6H2O.
Na2SeO3 0.001 G_PER_L TOGO M257 / JCM Medium 265 lists 0.001 g/L Na2SeO3.
Na2WO4 x 2H2O 0.01 G_PER_L TOGO M257 / JCM Medium 265 lists 0.01 g/L Na2WO4 x 2H2O.
JCM Medium 1068 lists 10.0 ml/L Wolfe's mineral solution.

8% NaHCO3 solution

Dose: 12.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaHCO3 8.0 PERCENT_W_V 8% NaHCO3 solution is represented from the stock label as 8.0% w/v NaHCO3.
JCM Medium 1068 adds 12.5 ml/L filter-sterilized 8% NaHCO3 solution after autoclaving.

0.2 M FeCl2 solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 0.2 MOLAR 0.2 M FeCl2 solution is represented from the stock label as 0.2 M FeCl2.
JCM Medium 1068 adds 10.0 ml/L freshly prepared and filter-sterilized 0.2 M FeCl2 solution after autoclaving.

Trace vitamins

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 2.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 2.0 mg/L Biotin.
Folic acid 2.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 2.0 mg/L Folic acid.
Pyridoxine HCl 10.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl.
Thiamine HCl 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Thiamine HCl.
Riboflavin 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Riboflavin.
Nicotinic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Nicotinic acid.
Calcium pantothenate 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Calcium pantothenate.
Vitamin B12 0.1 MG_PER_L TOGO M190 / JCM Medium 197 lists 0.1 mg/L Vitamin B12.
p-Aminobenzoic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid.
Lipoic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Lipoic acid.
Distilled water 1.0 L TOGO M190 / JCM Medium 197 lists 1.0 L Distilled water.
JCM Medium 1068 adds 10.0 ml/L filter-sterilized Trace vitamins after autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.989029Z togo-import Imported from TOGO Medium Source: JCM, ID: M1137
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas
2026-03-09T08:04:50.381679+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:19:50.402408Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007659
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T19:46:08.774379+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Saltwater Iron-Oxidizing Bacteria Medium-II" to "saltwater_iron_oxidizing_bacteria_medium_ii" for consistent matching
2026-05-16T09:03:07.823973+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-09-11T00:00:00-07:00 repair_togo_m1137_score15.py RESOLVED_TOGO_M1137_SCORE15 TOGO M1137 records JCM Medium 1068 as a saltwater iron-oxidizing bacteria medium. JCM 1068 mixes Artificial saltwater from JCM Medium 629 with Wolfe's mineral solution from JCM Medium 265, autoclaves the sealed vessels, aseptically adds filter-sterilized Trace vitamins from JCM Medium 197, 8% NaHCO3 solution, and freshly prepared 0.2 M FeCl2 solution, then replaces the gas phase with N2-CO2-O2 (80:20:3, v/v/v) and pressurizes it to 200 kPa.