microaerophilic_lutibacter_medium

CultureMech:007660 bacterial COMPLEX LIQUID
Original name: Microaerophilic Lutibacter Medium

Overview

External ontology
TOGO:M1138 — Microaerophilic Lutibacter Medium
Applications
  • Microbial cultivation
pH range
{'min': 6.2, 'max': 6.5}
Notes
TOGO M1138 records JCM Medium 1069 with Artificial saltwater from JCM Medium 629, MES, Wolfe's mineral solution from JCM Medium 265, Trace vitamins from JCM Medium 197, Tryptone, and an N2-CO2-O2 gas phase. JCM 1069 adjusts the medium to pH 6.2-6.5, distributes it into culture vessels, replaces the gas phase with N2-CO2-O2 (90:10:2, v/v/v), seals with butyl rubber stoppers, and autoclaves.

Composition

flowchart LR
    CultureMech_007660["microaerophilic_lutibacter_medium"]
    ing0_MES["MES"]
    CultureMech_007660 --> ing0_MES
    CHEBI_39010["CHEBI:39010"]
    ing0_MES -.-> CHEBI_39010
    ing1_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007660 --> ing1_Tryptone__BD_Difco_
    ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007660 --> ing2_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing2_Carbon_dioxide_gas -.-> CHEBI_16526
    ing3_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007660 --> ing3_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing3_Nitrogen_gas -.-> CHEBI_17997
    ing4_Oxygen_gas["Oxygen gas"]
    CultureMech_007660 --> ing4_Oxygen_gas
    CHEBI_15379["CHEBI:15379"]
    ing4_Oxygen_gas -.-> CHEBI_15379

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 MES 1.95 G_PER_L CHEBI:39010
MES
 TOGO M1138 / JCM Medium 1069 lists 1.95 g/L MES.
2 Tryptone (BD-Difco) 2.0 G_PER_L TOGO M1138 / JCM Medium 1069 lists 2.0 g/L Tryptone (BD-Difco).
3 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 JCM Medium 1069 replaces the gas phase with N2-CO2-O2 (90:10:2, v/v/v).
4 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 JCM Medium 1069 replaces the gas phase with N2-CO2-O2 (90:10:2, v/v/v).
5 Oxygen gas
O2 31.998 g/mol
variable VARIABLE CHEBI:15379
dioxygen
 JCM Medium 1069 replaces the gas phase with N2-CO2-O2 (90:10:2, v/v/v).

Preparation

  1. Mix Artificial saltwater, MES, Wolfe's mineral solution, Trace vitamins, and Tryptone.
  2. Adjust to pH 6.2-6.5.
  3. Distribute the medium into culture vessels, replace the gas phase with N2-CO2-O2 (90:10:2, v/v/v), and seal with butyl rubber stoppers.
  4. Autoclave at 121 degrees C for 15 min.

Solutions

Artificial saltwater

Dose: 1000.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaCl 27.5 G_PER_L TOGO M642 / JCM Medium 629 lists 27.5 g/L NaCl.
MgCl2 x 6H2O 5.38 G_PER_L TOGO M642 / JCM Medium 629 lists 5.38 g/L MgCl2 x 6H2O.
MgSO4 x 7H2O 6.78 G_PER_L TOGO M642 / JCM Medium 629 lists 6.78 g/L MgSO4 x 7H2O.
KCl 0.72 G_PER_L TOGO M642 / JCM Medium 629 lists 0.72 g/L KCl.
NaHCO3 0.2 G_PER_L TOGO M642 / JCM Medium 629 lists 0.2 g/L NaHCO3.
CaCl2 x 2H2O 1.4 G_PER_L TOGO M642 / JCM Medium 629 lists 1.4 g/L CaCl2 x 2H2O.
NH4Cl 1.0 G_PER_L TOGO M642 / JCM Medium 629 lists 1.0 g/L NH4Cl.
KH2PO4 0.05 G_PER_L TOGO M642 / JCM Medium 629 lists 0.05 g/L KH2PO4.
Distilled water 1.0 L TOGO M642 / JCM Medium 629 lists 1.0 L Distilled water.
JCM Medium 1069 lists 1.0 L Artificial saltwater.

Wolfe's mineral solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Nitrilotriacetic acid 1.5 G_PER_L JCM Medium 151 lists 1.5 g/L Nitrilotriacetic acid.
MgSO4 x 7H2O 3.0 G_PER_L JCM Medium 151 lists 3.0 g/L MgSO4 x 7H2O.
MnSO4 x xH2O 0.5 G_PER_L JCM Medium 151 lists 0.5 g/L MnSO4 x xH2O.
NaCl 1.0 G_PER_L JCM Medium 151 lists 1.0 g/L NaCl.
FeSO4 x 7H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L FeSO4 x 7H2O.
CoSO4 x 7H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L CoSO4 x 7H2O.
CaCl2 x 2H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L CaCl2 x 2H2O.
ZnSO4 x 7H2O 0.1 G_PER_L JCM Medium 151 lists 0.1 g/L ZnSO4 x 7H2O.
CuSO4 x 5H2O 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L CuSO4 x 5H2O.
AlK(SO4)2 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L AlK(SO4)2.
H3BO3 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L H3BO3.
Na2MoO4 x 2H2O 0.01 G_PER_L JCM Medium 151 lists 0.01 g/L Na2MoO4 x 2H2O.
Distilled water 1.0 L JCM Medium 151 lists 1.0 L Distilled water.
NiCl2 x 6H2O 0.02 G_PER_L TOGO M257 / JCM Medium 265 lists 0.02 g/L NiCl2 x 6H2O.
Na2SeO3 0.001 G_PER_L TOGO M257 / JCM Medium 265 lists 0.001 g/L Na2SeO3.
Na2WO4 x 2H2O 0.01 G_PER_L TOGO M257 / JCM Medium 265 lists 0.01 g/L Na2WO4 x 2H2O.
JCM Medium 1069 lists 1.0 ml/L Wolfe's mineral solution.

Trace vitamins

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 2.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 2.0 mg/L Biotin.
Folic acid 2.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 2.0 mg/L Folic acid.
Pyridoxine HCl 10.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl.
Thiamine HCl 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Thiamine HCl.
Riboflavin 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Riboflavin.
Nicotinic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Nicotinic acid.
Calcium pantothenate 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Calcium pantothenate.
Vitamin B12 0.1 MG_PER_L TOGO M190 / JCM Medium 197 lists 0.1 mg/L Vitamin B12.
p-Aminobenzoic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid.
Lipoic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Lipoic acid.
Distilled water 1.0 L TOGO M190 / JCM Medium 197 lists 1.0 L Distilled water.
JCM Medium 1069 lists 10.0 ml/L Trace vitamins.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.990104Z togo-import Imported from TOGO Medium Source: JCM, ID: M1138
2026-01-28T19:48:19.321892Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas
2026-03-09T08:04:31.992102+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:50.407420Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007660
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.779465+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Microaerophilic Lutibacter Medium" to "microaerophilic_lutibacter_medium" for consistent matching
2026-05-16T09:03:07.829554+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.696007+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1138_score15.py RESOLVED_TOGO_M1138_SCORE15 TOGO M1138 records JCM Medium 1069 with Artificial saltwater from JCM Medium 629, MES, Wolfe's mineral solution from JCM Medium 265, Trace vitamins from JCM Medium 197, Tryptone, and an N2-CO2-O2 gas phase. JCM 1069 adjusts the medium to pH 6.2-6.5, distributes it into culture vessels, replaces the gas phase with N2-CO2-O2 (90:10:2, v/v/v), seals with butyl rubber stoppers, and autoclaves.