cuniculiplasma_medium

CultureMech:007667 archaea COMPLEX LIQUID
Original name: Cuniculiplasma Medium

Overview

External ontology
TOGO:M1144 — Cuniculiplasma Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1144 Original source: JCM - JCM_M1075 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1075

Composition

flowchart LR
    CultureMech_007667["cuniculiplasma_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007667 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007667 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007667 --> ing2_Yeast_extract
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007667 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_007667 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007667 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_FeCl3_6H2O["FeCl3・6H2O"]
    CultureMech_007667 --> ing6_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_6H2O -.-> CHEBI_86254
    ing7_Betaine["Betaine"]
    CultureMech_007667 --> ing7_Betaine
    CHEBI_17750["CHEBI:17750"]
    ing7_Betaine -.-> CHEBI_17750
    ing8_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_007667 --> ing8_Beef_extract__BD_Difco_
    ing9_Tryptone["Tryptone"]
    CultureMech_007667 --> ing9_Tryptone
    ing10_H2SO4["H2SO4"]
    CultureMech_007667 --> ing10_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing10_H2SO4 -.-> CHEBI_26836

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 0.1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
7 FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 Betaine
C5H11NO2 117.148 g/mol
0.6 G_PER_L CHEBI:17750
glycine betaine
 Role: Growth factor; Properties: Defined component, Simple component, Organic compound
9 Beef extract (BD-Difco) 1 G_PER_L Properties: Undefined component
10 Tryptone 1 G_PER_L Properties: Undefined component
11 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Solutions

Kao and Michayluk vitamin solution (Sigma--Aldrich)

Dose: 10 G_PER_L

Role: Growth factor; Properties: Complex component, Solution

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.002209Z togo-import Imported from TOGO Medium Source: JCM, ID: M1144
2026-01-28T19:48:38.278621Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-09T08:05:04.043584+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:50.463257Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007667
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.836786+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cuniculiplasma Medium" to "cuniculiplasma_medium" for consistent matching
2026-05-16T09:03:07.876372+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.749197+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.462087+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.021239+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.