alkaliphilus_lacv_medium

CultureMech:007669 bacterial COMPLEX LIQUID
Original name: Alkaliphilus LACV Medium

Overview

External ontology
TOGO:M1146 — Alkaliphilus LACV Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1146 Original source: JCM - JCM_M1077 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1077

Composition

flowchart LR
    CultureMech_007669["alkaliphilus_lacv_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007669 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007669 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007669 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007669 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2HPO4["K2HPO4"]
    CultureMech_007669 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_007669 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007669 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_007669 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Tris_base["Tris base"]
    CultureMech_007669 --> ing8_Tris_base
    CHEBI_9754["CHEBI:9754"]
    ing8_Tris_base -.-> CHEBI_9754
    ing9_Na2S2O3_5H2O["Na2S2O3・5H2O"]
    CultureMech_007669 --> ing9_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing9_Na2S2O3_5H2O -.-> CHEBI_32150
    ing10_NaOH["NaOH"]
    CultureMech_007669 --> ing10_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing10_NaOH -.-> CHEBI_32145
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007669 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_N2["N2"]
    CultureMech_007669 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997
    ing13_0_5_M_Crotonic_acid__pH_7_0_["0.5 M Crotonic acid (pH 7.0)"]
    CultureMech_007669 --> ing13_0_5_M_Crotonic_acid__pH_7_0_

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
940 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5 K2HPO4
HO4P.2K 174.174 g/mol
0.1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 Tris base
C4H11NO3 121.136 g/mol
3 G_PER_L CHEBI:9754
tris
 Role: Buffer; Properties: Defined component, Simple component, Organic compound
10 Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
3 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
 Properties: Defined component, Inorganic compound, Simple component
11 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
12 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: Gas
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
14 0.5 M Crotonic acid (pH 7.0) 20 G_PER_L Role: Carbon source; Properties: Solution, Organic compound, Defined component

Solutions

Trace element solution (see Medium [M439])

Dose: 1 G_PER_L

Cross-reference to Medium M439

8% Na2CO3 solution

Dose: 20 G_PER_L

Role: Buffer; Properties: Defined component, Solution, Inorganic compound

10% (w/v) Yeast extract solution

Dose: 20 G_PER_L

Role: Nutrient source; Properties: Undefined component

5% Na2S・9H2O solution

Dose: 6 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.006200Z togo-import Imported from TOGO Medium Source: JCM, ID: M1146
2026-01-28T19:48:40.896027Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2
2026-03-09T08:05:08.842160+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T06:19:50.482310Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007669
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.856095+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaliphilus LACV Medium" to "alkaliphilus_lacv_medium" for consistent matching
2026-05-16T09:03:07.897790+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.771203+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.029524+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.