halanaeroarchaeum_medium

CultureMech:007671 archaea COMPLEX LIQUID
Original name: Halanaeroarchaeum Medium

Overview

External ontology
TOGO:M1148 — Halanaeroarchaeum Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1148 Original source: JCM - JCM_M1079 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1079

Composition

flowchart LR
    CultureMech_007671["halanaeroarchaeum_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007671 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007671 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NH4Cl["NH4Cl"]
    CultureMech_007671 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_K2HPO4["K2HPO4"]
    CultureMech_007671 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_HEPES["HEPES"]
    CultureMech_007671 --> ing4_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing4_HEPES -.-> CHEBI_46756
    ing5_Na2MoO4_2H2O["Na2MoO4・2H2O"]
    CultureMech_007671 --> ing5_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing5_Na2MoO4_2H2O -.-> CHEBI_75213
    ing6_H3BO3["H3BO3"]
    CultureMech_007671 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_007671 --> ing7_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_7H2O -.-> CHEBI_75836
    ing8_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_007671 --> ing8_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_4H2O -.-> CHEBI_86368
    ing9_CoCl2_6H2O["CoCl2・6H2O"]
    CultureMech_007671 --> ing9_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing9_CoCl2_6H2O -.-> CHEBI_35696
    ing10_NiCl2_6H2O["NiCl2・6H2O"]
    CultureMech_007671 --> ing10_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_6H2O -.-> CHEBI_34887
    ing11_ZnSO4_7H2O["ZnSO4・7H2O"]
    CultureMech_007671 --> ing11_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_7H2O -.-> CHEBI_32312
    ing12_CuCl2_2H2O["CuCl2・2H2O"]
    CultureMech_007671 --> ing12_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_2H2O -.-> CHEBI_86318
    ing13_EDTA["EDTA"]
    CultureMech_007671 --> ing13_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing13_EDTA -.-> CHEBI_4735

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0]
2 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
4 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 HEPES 7 G_PER_L CHEBI:46756
HEPES
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
6 Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 CoCl2・6H2O
2Cl.Co 129.839 g/mol
0.2 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 NiCl2・6H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
 Properties: Defined component, Organic compound, Simple component

Solutions

1 M NaHCO3 solution*

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Solution, Inorganic compound

1 M MgCl2 solution

Dose: 1 G_PER_L

Role: Mineral source

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

Trace element solution (see below)

Dose: 1 G_PER_L

10% Yeast extract solution

Dose: 0.2 G_PER_L

Role: Nutrient source; Properties: Undefined component

2 M Sodium pyruvate solution*

Dose: 10 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

5% Na2S・9H2O solution

Dose: 5 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.010676Z togo-import Imported from TOGO Medium Source: JCM, ID: M1148
2026-01-28T19:48:08.178155Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:13.160835+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T03:08:40.120122Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:50.502571Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007671
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.876746+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halanaeroarchaeum Medium" to "halanaeroarchaeum_medium" for consistent matching
2026-05-16T09:03:07.921072+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:30.795049+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.038699+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:25.628738+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.