halodesulfuriarchaeum_medium

CultureMech:007672 bacterial COMPLEX LIQUID
Original name: Halodesulfuriarchaeum Medium

Overview

External ontology
TOGO:M1149 — Halodesulfuriarchaeum Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1149 Original source: JCM - JCM_M1080 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1080

Composition

flowchart LR
    CultureMech_007672["halodesulfuriarchaeum_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007672 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007672 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NH4Cl["NH4Cl"]
    CultureMech_007672 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_K2HPO4["K2HPO4"]
    CultureMech_007672 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_HEPES["HEPES"]
    CultureMech_007672 --> ing4_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing4_HEPES -.-> CHEBI_46756
    ing5_yeast_extract["yeast extract"]
    CultureMech_007672 --> ing5_yeast_extract
    ing6_sodium_formate["sodium formate"]
    CultureMech_007672 --> ing6_sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing6_sodium_formate -.-> CHEBI_62965

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
4 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
5 HEPES 7 G_PER_L CHEBI:46756
HEPES
 Role: Buffer; Properties: Organic compound, Simple component, Defined component
6 yeast extract 200 MG_PER_L Role: Nutrient source; Properties: Undefined component
7 sodium formate
CHO2.Na 68.007 g/mol
variable VARIABLE CHEBI:62965
sodium formate
 Properties: Defined component, Organic compound, Solution

Solutions

1 M NaHCO3 solution*

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1 M MgCl2 solution

Dose: 1 G_PER_L

Role: Mineral source

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

Trace element solution (see Medium [M1148])

Dose: 1 G_PER_L

Cross-reference to Medium M1148

5% Na2S・9H2O solution

Dose: 5 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.013468Z togo-import Imported from TOGO Medium Source: JCM, ID: M1149
2026-01-28T19:48:35.290573Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: sodium formate
2026-03-09T08:04:58.689549+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:19:50.511272Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007672
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.885677+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halodesulfuriarchaeum Medium" to "halodesulfuriarchaeum_medium" for consistent matching
2026-05-16T09:03:07.931294+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.805344+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.043680+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.