Overview
- External ontology
- TOGO:M1150 — Natranaeroarchaeum Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- JCM Medium 1081 composes Natranaeroarchaeum Medium from sterilized JCM 1079 Basal mineral NaCl medium, JCM 1081 Soda based mineral medium, 2 M sodium pyruvate, Trace vitamins from JCM 197, and 10% yeast extract.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare and sterilize Basal mineral NaCl medium and Soda based mineral medium.
- Mix 250 ml Basal mineral NaCl medium with 750 ml Soda based mineral medium.
- Aseptically add sodium pyruvate, Trace vitamins, and yeast extract solutions.
Solutions
Basal mineral NaCl medium
Dose: 250.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 240.0 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| K2HPO4 | 2.5 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| NH4Cl | 0.5 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| HEPES | 7.0 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 1079 prints this component in its stock solution. |
JCM Medium 1081 adds 250.0 ml/L sterilized Basal mineral NaCl medium from JCM Medium 1079.
Soda based mineral medium
Dose: 750.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 185.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| NaHCO3 | 35.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| NaCl | 16.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| K2HPO4 | 1.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| KCl | 5.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| 4 M NH4Cl solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L 4 M NH4Cl solution to the soda based mineral medium. |
| Selenite-tungstate solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L JCM 431 Selenite-tungstate solution. |
| Trace element solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L JCM 1079 Trace element solution. |
| 1 M MgCl2 solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L 1 M MgCl2 solution to the soda based mineral medium. |
| Distilled water | 1.0 L | JCM Medium 1081 prints this component in its stock solution. |
JCM Medium 1081 adds 750.0 ml/L sterilized Soda based mineral medium from JCM Medium 1081.
2 M Sodium pyruvate solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium pyruvate | 2.0 MOLAR | JCM Medium 1081 identifies this as a 2.0 M stock solution. |
JCM Medium 1081 adds 5.0 ml/L filter-sterilized 2 M Sodium pyruvate solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 197 prints this component in its stock solution. |
JCM Medium 1081 adds 10.0 ml/L Trace vitamins from JCM Medium 197.
10% Yeast extract solution
Dose: 0.2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 10 PERCENT_W_V | JCM Medium 1081 identifies this as a 10% w/v stock solution. |
JCM Medium 1081 adds 0.2 ml/L 10% Yeast extract solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.016728Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1150 |
| 2026-01-28T19:48:09.934984Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:16.155995+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T06:19:50.524787Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007674 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.900397+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natranaeroarchaeum Medium" to "natranaeroarchaeum_medium" for consistent matching |
| 2026-05-16T09:03:07.946669+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.821811+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.047227+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | JCM Medium 1081 composes Natranaeroarchaeum Medium from sterilized JCM 1079 Basal mineral NaCl medium, JCM 1081 Soda based mineral medium, 2 M sodium pyruvate, Trace vitamins from JCM 197, and 10% yeast extract. |