Overview
- External ontology
- TOGO:M1155 — Peat Medium With Sucrose
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1155 records JCM Medium 1086 with peptone, yeast extract, sucrose, Major metals from JCM Medium 923, Trace metal 1 solution from JCM Medium 923, distilled water, filter-sterilized 0.5 M MES solution at pH 5.7, and an N2-CO2 (4:1, v/v) gas phase. JCM 1086 autoclaves the base medium at 121 degrees C for 15 min, adds filter-sterilized MES after cooling, and then distributes it aseptically into culture vessels under N2-CO2.
Composition
flowchart LR
CultureMech_007679["peat_medium_with_sucrose"]
ing0_Peptone__BD_Difco_["Peptone (BD-Difco)"]
CultureMech_007679 --> ing0_Peptone__BD_Difco_
ing1_Yeast_extract["Yeast extract"]
CultureMech_007679 --> ing1_Yeast_extract
ing2_Sucrose["Sucrose"]
CultureMech_007679 --> ing2_Sucrose
CHEBI_17992["CHEBI:17992"]
ing2_Sucrose -.-> CHEBI_17992
ing3_Distilled_water["Distilled water"]
CultureMech_007679 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007679 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_Nitrogen_gas["Nitrogen gas"]
CultureMech_007679 --> ing5_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing5_Nitrogen_gas -.-> CHEBI_17997
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone (BD-Difco) | 0.25 G_PER_L | JCM Medium 1086 lists 0.25 g/L Peptone (BD-Difco). | |
| 2 | Yeast extract | 1.0 G_PER_L | JCM Medium 1086 lists 1.0 g/L Yeast extract. | |
| 3 |
Sucrose
C12H22O11 342.297 g/mol
|
2.0 G_PER_L |
CHEBI:17992
sucrose
|
JCM Medium 1086 lists 2.0 g/L Sucrose. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 1086 lists 1.0 L Distilled water. |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 1086 replaces the gas phase with N2-CO2 (4:1, v/v). |
| 6 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1086 replaces the gas phase with N2-CO2 (4:1, v/v). |
Preparation
- Mix peptone, yeast extract, sucrose, Major metals, Trace metal 1 solution, and distilled water thoroughly.
- Autoclave the base medium at 121 degrees C for 15 min.
- Cool the autoclaved base medium, then add filter-sterilized 0.5 M MES solution.
- Aseptically distribute the medium into culture vessels and replace the gas phase with N2-CO2 (4:1, v/v).
Solutions
Major metals
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KCl | 0.15 G_PER_L | JCM Medium 923 lists 0.15 g/L KCl in this stock solution. |
| KH2PO4 | 1.36 G_PER_L | JCM Medium 923 lists 1.36 g/L KH2PO4 in this stock solution. |
| NH4Cl | 2.68 G_PER_L | JCM Medium 923 lists 2.68 g/L NH4Cl in this stock solution. |
| Distilled water | 1.0 L | JCM Medium 923 lists 1.0 L Distilled water in this stock solution. |
JCM Medium 1086 adds 10.0 ml/L Major metals from JCM Medium 923.
Trace metal 1 solution
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CoCl2 x 6H2O | 24.0 MG_PER_L | JCM Medium 923 lists 24.0 mg/L CoCl2 x 6H2O in this stock solution. |
| ZnCl2 | 75.0 MG_PER_L | JCM Medium 923 lists 75.0 mg/L ZnCl2 in this stock solution. |
| H3BO3 | 19.0 MG_PER_L | JCM Medium 923 lists 19.0 mg/L H3BO3 in this stock solution. |
| NiCl2 x 6H2O | 24.0 MG_PER_L | JCM Medium 923 lists 24.0 mg/L NiCl2 x 6H2O in this stock solution. |
| Na2MoO4 x 2H2O | 24.0 MG_PER_L | JCM Medium 923 lists 24.0 mg/L Na2MoO4 x 2H2O in this stock solution. |
| FeCl2 x 4H2O | 1.344 G_PER_L | JCM Medium 923 lists 1.344 g/L FeCl2 x 4H2O in this stock solution. |
| MnSO4 x H2O | 26.0 MG_PER_L | JCM Medium 923 lists 26.0 mg/L MnSO4 x H2O in this stock solution. |
| MgSO4 x 7H2O | 1.556 G_PER_L | JCM Medium 923 lists 1.556 g/L MgSO4 x 7H2O in this stock solution. |
| CaCl2 x 2H2O | 2.336 G_PER_L | JCM Medium 923 lists 2.336 g/L CaCl2 x 2H2O in this stock solution. |
| CuSO4 x 5H2O | 9.0 MG_PER_L | JCM Medium 923 lists 9.0 mg/L CuSO4 x 5H2O in this stock solution. |
| AlK(SO4)2 x 12H2O | 3.446 G_PER_L | JCM Medium 923 lists 3.446 g/L AlK(SO4)2 x 12H2O in this stock solution. |
| Distilled water | 1.0 L | JCM Medium 923 lists 1.0 L Distilled water in this stock solution. |
JCM Medium 1086 adds 0.1 ml/L Trace metal 1 solution from JCM Medium 923.
0.5 M MES solution (pH 5.7)
Dose: 40.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MES | 0.5 MOLAR | JCM Medium 1086 identifies this as a 0.5 M MES stock solution. |
JCM Medium 1086 adds 40.0 ml/L 0.5 M MES solution at pH 5.7 after autoclaving and cooling.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.025775Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1155 |
| 2026-01-28T19:48:18.984773Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:31.445940+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:50.563333Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007679 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.960232+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Peat Medium With Sucrose" to "peat_medium_with_sucrose" for consistent matching |
| 2026-05-16T09:03:07.992277+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.864291+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1155_score15.py | RESOLVED_TOGO_M1155_SCORE15 | TOGO M1155 records JCM Medium 1086 with peptone, yeast extract, sucrose, Major metals from JCM Medium 923, Trace metal 1 solution from JCM Medium 923, distilled water, filter-sterilized 0.5 M MES solution at pH 5.7, and an N2-CO2 (4:1, v/v) gas phase. JCM 1086 autoclaves the base medium at 121 degrees C for 15 min, adds filter-sterilized MES after cooling, and then distributes it aseptically into culture vessels under N2-CO2. |