briggs_liver_broth_ph_5_0

CultureMech:007683 bacterial COMPLEX LIQUID
Original name: Briggs Liver Broth (pH 5.0)

Overview

External ontology
TOGO:M115 — Briggs Liver Broth (pH 5.0)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M115 Original source: JCM - JCM_M123 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=123

Composition

flowchart LR
    CultureMech_007683["briggs_liver_broth_ph_5_0"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007683 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007683 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Tween_80["Tween 80"]
    CultureMech_007683 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_Glucose["Glucose"]
    CultureMech_007683 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Soluble_starch["Soluble starch"]
    CultureMech_007683 --> ing4_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Soluble_starch -.-> CHEBI_28017
    ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007683 --> ing5_Yeast_extract__BD_Difco_
    ing6_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007683 --> ing6_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing7_Neopeptone__BD_Difco_["Neopeptone (BD-Difco)"]
    CultureMech_007683 --> ing7_Neopeptone__BD_Difco_
    ing8_Tomato_juice__Del_Monte_["Tomato juice (Del Monte)"]
    CultureMech_007683 --> ing8_Tomato_juice__Del_Monte_
    ing9_NaOH["NaOH"]
    CultureMech_007683 --> ing9_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing9_NaOH -.-> CHEBI_32145

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
725.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 525.0, 200.0]
2 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Defined component, Organic compound, Simple component
4 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
5 Soluble starch 0.5 G_PER_L CHEBI:28017
starch
 Role: Carbon source; Properties: Undefined component
6 Yeast extract (BD-Difco) 6 G_PER_L Properties: Undefined component
7 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.2 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Organic compound, Simple component, Defined component
8 Neopeptone (BD-Difco) 15 G_PER_L Properties: Undefined component
9 Tomato juice (Del Monte) 200 G_PER_L Role: Growth factor; Properties: Undefined component
10 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution

Solutions

Tomato juice solution (see below)

Dose: 400 G_PER_L

Liver extract (see Medium [M6])

Dose: 75 G_PER_L

Cross-reference to Medium M6

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.015075Z togo-import Imported from TOGO Medium Source: JCM, ID: M115
2026-01-28T19:48:36.939466Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-09T08:05:01.648118+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:09:33.487539Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:50.588087Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007683
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.986454+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Briggs Liver Broth (pH 5.0)" to "briggs_liver_broth_ph_5_0" for consistent matching
2026-05-16T09:03:08.021866+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.891725+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.