Overview
- External ontology
- TOGO:M1168 — Bicarbonate Buffered Medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1168 records JCM Medium 1095 with a bicarbonate-buffered base containing FeCl2 and Trace element stocks from JCM Medium 187, Selenite-tungstate solution from JCM Medium 431, filter-sterilized Trace vitamins from JCM Medium 197, filter-sterilized 8% NaHCO3, 10% MgCl2 x 6H2O, 10% CaCl2 x 2H2O, 5% Na2S x 9H2O, 1 M maltose solution, and an N2-CO2 (4:1, v/v) gas phase.
Composition
flowchart LR
CultureMech_007692["bicarbonate_buffered_medium"]
ing0_Na2HPO4_x_2H2O["Na2HPO4 x 2H2O"]
CultureMech_007692 --> ing0_Na2HPO4_x_2H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_007692 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_007692 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_007692 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Yeast_extract["Yeast extract"]
CultureMech_007692 --> ing4_Yeast_extract
ing5_Resazurin["Resazurin"]
CultureMech_007692 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Distilled_water["Distilled water"]
CultureMech_007692 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007692 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_007692 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.53 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
JCM Medium 1095 lists 0.53 g/L Na2HPO4 x 2H2O. |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 1095 lists 0.41 g/L KH2PO4. |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 1095 lists 0.3 g/L NH4Cl. |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.3 G_PER_L |
CHEBI:26710
sodium chloride
|
JCM Medium 1095 lists 0.3 g/L NaCl. |
| 5 | Yeast extract | 0.1 G_PER_L | JCM Medium 1095 lists 0.1 g/L Yeast extract. | |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
JCM Medium 1095 lists 0.5 mg/L Resazurin. |
| 7 |
Distilled water
H2O 18.015 g/mol
|
930.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1095 lists 930.0 ml/L Distilled water. |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 1095 uses an N2-CO2 (4:1, v/v) gas mixture. |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1095 uses an N2-CO2 (4:1, v/v) gas mixture. |
Preparation
- Mix the salts, yeast extract, Resazurin, FeCl2 solution, Trace element solution, Selenite-tungstate solution, and distilled water.
- Autoclave the base medium under an N2-CO2 (4:1, v/v) gas mixture.
- After cooling, add MgCl2, CaCl2, Trace vitamins, NaHCO3, and Substrate solutions.
- Aseptically and anaerobically distribute medium into culture vessels under N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers.
- Prior to use, add 10.0 ml/L 5% Na2S x 9H2O solution autoclaved and stored under N2.
Solutions
FeCl2 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| HCl | 10.0 ML_PER_L | JCM Medium 187 lists 10.0 ml/L HCl. |
| FeCl2 x 4H2O | 1.5 G_PER_L | JCM Medium 187 lists 1.5 g/L FeCl2 x 4H2O. |
| Distilled water | 990.0 ML_PER_L | JCM Medium 187 lists 990.0 ml/L Distilled water. |
JCM Medium 1095 adds 1.0 ml/L FeCl2 solution from JCM Medium 187.
Trace element solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnCl2 | 70.0 MG_PER_L | JCM Medium 187 lists 70.0 mg/L ZnCl2. |
| MnCl2 x 4H2O | 100.0 MG_PER_L | JCM Medium 187 lists 100.0 mg/L MnCl2 x 4H2O. |
| H3BO3 | 6.0 MG_PER_L | JCM Medium 187 lists 6.0 mg/L H3BO3. |
| CoCl2 x 6H2O | 190.0 MG_PER_L | JCM Medium 187 lists 190.0 mg/L CoCl2 x 6H2O. |
| CuCl2 x 2H2O | 2.0 MG_PER_L | JCM Medium 187 lists 2.0 mg/L CuCl2 x 2H2O. |
| NiCl2 x 6H2O | 24.0 MG_PER_L | JCM Medium 187 lists 24.0 mg/L NiCl2 x 6H2O. |
| Na2MoO4 x 2H2O | 36.0 MG_PER_L | JCM Medium 187 lists 36.0 mg/L Na2MoO4 x 2H2O. |
| Distilled water | 1.0 L | JCM Medium 187 lists 1.0 L Distilled water. |
JCM Medium 1095 adds 1.0 ml/L Trace element solution from JCM Medium 187.
Selenite-tungstate solution
Dose: 0.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | 0.4 G_PER_L | JCM Medium 431 lists 0.4 g/L NaOH. |
| Na2SeO3 x 5H2O | 6.0 MG_PER_L | JCM Medium 431 lists 6.0 mg/L Na2SeO3 x 5H2O. |
| Na2WO4 x 2H2O | 8.0 MG_PER_L | JCM Medium 431 lists 8.0 mg/L Na2WO4 x 2H2O. |
| Distilled water | 1.0 L | JCM Medium 431 lists 1.0 L Distilled water. |
JCM Medium 1095 adds 0.5 ml/L Selenite-tungstate solution from JCM Medium 431.
10% MgCl2 x 6H2O solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 x 6H2O | 10.0 PERCENT_W_V | 10% MgCl2 x 6H2O solution is represented from the stock label as 10.0% w/v MgCl2 x 6H2O. |
JCM Medium 1095 adds 1.0 ml/L 10% MgCl2 x 6H2O solution.
10% CaCl2 x 2H2O solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2H2O | 10.0 PERCENT_W_V | 10% CaCl2 x 2H2O solution is represented from the stock label as 10.0% w/v CaCl2 x 2H2O. |
JCM Medium 1095 adds 1.0 ml/L 10% CaCl2 x 2H2O solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | JCM Medium 197 lists 1.0 L Distilled water. |
JCM Medium 1095 adds 10.0 ml/L filter-sterilized Trace vitamins.
8% NaHCO3 solution
Dose: 50.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 8.0 PERCENT_W_V | 8% NaHCO3 solution is represented from the stock label as 8.0% w/v NaHCO3. |
JCM Medium 1095 adds 50.0 ml/L 8% NaHCO3 solution.
1 M maltose solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Maltose | 1.0 MOLAR | JCM Medium 1095 lists 1 M maltose solution as a Substrate solution option. |
JCM Medium 1095 adds 10.0 ml/L Substrate solution and lists 1 M maltose solution as this substrate option.
5% Na2S x 9H2O solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 5.0 PERCENT_W_V | 5% Na2S x 9H2O solution is represented from the stock label as 5.0% w/v Na2S x 9H2O. |
JCM Medium 1095 adds 10.0 ml/L 5% Na2S x 9H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.046326Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1168 |
| 2026-01-28T19:48:07.573354Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: maltose solution |
| 2026-03-09T08:04:12.208141+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 10 solution(s) from ingredients |
| 2026-03-13T03:08:39.034820Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:50.657896Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007692 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.058989+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Bicarbonate Buffered Medium" to "bicarbonate_buffered_medium" for consistent matching |
| 2026-05-16T09:03:08.095143+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.970268+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.094358+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1165_m1168_score15.py | RESOLVED_TOGO_M1165_M1168_SCORE15 | TOGO M1168 records JCM Medium 1095 with a bicarbonate-buffered base containing FeCl2 and Trace element stocks from JCM Medium 187, Selenite-tungstate solution from JCM Medium 431, filter-sterilized Trace vitamins from JCM Medium 197, filter-sterilized 8% NaHCO3, 10% MgCl2 x 6H2O, 10% CaCl2 x 2H2O, 5% Na2S x 9H2O, 1 M maltose solution, and an N2-CO2 (4:1, v/v) gas phase. |