Overview
- External ontology
- TOGO:M1171 — BSM-RC Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1171 Original source: JCM - JCM_M1098 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1098
Composition
flowchart LR
CultureMech_007696["bsm_rc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007696 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007696 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_007696 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_NH4Cl["NH4Cl"]
CultureMech_007696 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2HPO4["K2HPO4"]
CultureMech_007696 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_NaNO3["NaNO3"]
CultureMech_007696 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_Na2SO4["Na2SO4"]
CultureMech_007696 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_L__Sodium_lactate["L--Sodium lactate"]
CultureMech_007696 --> ing7_L__Sodium_lactate
ing8_Tryptone["Tryptone"]
CultureMech_007696 --> ing8_Tryptone
ing9_N2["N2"]
CultureMech_007696 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_007696 --> ing10_Nitrogen_gas
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_Oxygen_gas["Oxygen gas"]
CultureMech_007696 --> ing11_Oxygen_gas
ing12_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007696 --> ing12_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_2H2O -.-> CHEBI_86158
ing13_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007696 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007696 --> ing14_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing14_MgCl2_6H2O -.-> CHEBI_86345
ing15_H3BO3["H3BO3"]
CultureMech_007696 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007696 --> ing16_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_4H2O -.-> CHEBI_86368
ing17_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_007696 --> ing17_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing17_CoCl2_6H2O -.-> CHEBI_35696
ing18_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_007696 --> ing18_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_6H2O -.-> CHEBI_34887
ing19_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_007696 --> ing19_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_2H2O -.-> CHEBI_86318
ing20_ZnCl2["ZnCl2"]
CultureMech_007696 --> ing20_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing20_ZnCl2 -.-> CHEBI_49976
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
936.5 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 935.5, 1.0] |
| 2 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
3.62 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.075 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.26 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.85 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.05 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | L--Sodium lactate | 2.24 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 9 | Tryptone | 0.5 G_PER_L | Properties: Undefined component | |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 | Oxygen gas | variable VARIABLE | Properties: Gas | |
| 13 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
100 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see below)
Dose: 1 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
8% NaHCO3 solution
Dose: 52.5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.054385Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1171 |
| 2026-01-28T19:48:35.824582Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:59.653014+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:31.408786Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:50.695499Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007696 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.098393+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSM-RC Medium" to "bsm_rc_medium" for consistent matching |
| 2026-05-16T09:03:08.135204+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.015374+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.111579+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |