Overview
- External ontology
- TOGO:M1173 — CTM Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1173 Original source: JCM - JCM_M1100 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1100
Composition
flowchart LR
CultureMech_007698["ctm_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007698 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_HEPES["HEPES"]
CultureMech_007698 --> ing1_HEPES
CHEBI_46756["CHEBI:46756"]
ing1_HEPES -.-> CHEBI_46756
ing2_KOH["KOH"]
CultureMech_007698 --> ing2_KOH
CHEBI_32035["CHEBI:32035"]
ing2_KOH -.-> CHEBI_32035
ing3_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007698 --> ing3_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_7H2O -.-> CHEBI_31795
ing4_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007698 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_Citric_acid["Citric acid"]
CultureMech_007698 --> ing5_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing5_Citric_acid -.-> CHEBI_30769
ing6_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007698 --> ing6_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_2H2O -.-> CHEBI_75213
ing7_H3BO3["H3BO3"]
CultureMech_007698 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007698 --> ing8_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_4H2O -.-> CHEBI_86368
ing9_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007698 --> ing9_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_7H2O -.-> CHEBI_32312
ing10_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_007698 --> ing10_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_5H2O -.-> CHEBI_31440
ing11_Co_NO3_2_6H2O["Co(NO3)2・6H2O"]
CultureMech_007698 --> ing11_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing11_Co_NO3_2_6H2O -.-> CHEBI_86214
ing12_K2HPO4["K2HPO4"]
CultureMech_007698 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_007698 --> ing13_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing13_Ferric_ammonium_citrate -.-> CHEBI_31604
ing14_2_Oxoglutarate["2-Oxoglutarate"]
CultureMech_007698 --> ing14_2_Oxoglutarate
CHEBI_16810["CHEBI:16810"]
ing14_2_Oxoglutarate -.-> CHEBI_16810
ing15_NaHCO3["NaHCO3"]
CultureMech_007698 --> ing15_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing15_NaHCO3 -.-> CHEBI_32139
ing16_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_007698 --> ing16_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing16_Sodium_thioglycolate -.-> CHEBI_86481
ing17_Bacto_peptone__BD__Difco_["Bacto peptone (BD--Difco)"]
CultureMech_007698 --> ing17_Bacto_peptone__BD__Difco_
ing18_L__Cysteine["L--Cysteine"]
CultureMech_007698 --> ing18_L__Cysteine
CHEBI_17561["CHEBI:17561"]
ing18_L__Cysteine -.-> CHEBI_17561
ing19_Glycine["Glycine"]
CultureMech_007698 --> ing19_Glycine
CHEBI_15428["CHEBI:15428"]
ing19_Glycine -.-> CHEBI_15428
ing20_L__Glutamic_acid["L--Glutamic acid"]
CultureMech_007698 --> ing20_L__Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing20_L__Glutamic_acid -.-> CHEBI_16015
ing21_L__Alanine["L--Alanine"]
CultureMech_007698 --> ing21_L__Alanine
CHEBI_16977["CHEBI:16977"]
ing21_L__Alanine -.-> CHEBI_16977
ing22_L__Arginine["L--Arginine"]
CultureMech_007698 --> ing22_L__Arginine
CHEBI_16467["CHEBI:16467"]
ing22_L__Arginine -.-> CHEBI_16467
ing23_L__Aspartic_acid["L--Aspartic acid"]
CultureMech_007698 --> ing23_L__Aspartic_acid
CHEBI_17053["CHEBI:17053"]
ing23_L__Aspartic_acid -.-> CHEBI_17053
ing24_L__Glutamine["L--Glutamine"]
CultureMech_007698 --> ing24_L__Glutamine
CHEBI_18050["CHEBI:18050"]
ing24_L__Glutamine -.-> CHEBI_18050
ing25_L__Isoleucine["L--Isoleucine"]
CultureMech_007698 --> ing25_L__Isoleucine
CHEBI_17191["CHEBI:17191"]
ing25_L__Isoleucine -.-> CHEBI_17191
ing26_L__Leucine["L--Leucine"]
CultureMech_007698 --> ing26_L__Leucine
CHEBI_15603["CHEBI:15603"]
ing26_L__Leucine -.-> CHEBI_15603
ing27_L__Lysine["L--Lysine"]
CultureMech_007698 --> ing27_L__Lysine
CHEBI_18019["CHEBI:18019"]
ing27_L__Lysine -.-> CHEBI_18019
ing28_L__Phenylalanine["L--Phenylalanine"]
CultureMech_007698 --> ing28_L__Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing28_L__Phenylalanine -.-> CHEBI_17295
ing29_L__Proline["L--Proline"]
CultureMech_007698 --> ing29_L__Proline
CHEBI_17203["CHEBI:17203"]
ing29_L__Proline -.-> CHEBI_17203
CultureMech_007698 --> more["...23 more"]
Ingredients (53)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
625.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 10 duplicates: 1.0, 1.0, 1.0, 1.0, 1.0, 1.0, 19.0, 100.0, 400.0, 100.0] |
| 2 | HEPES | 2.4 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 3 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 4 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.75 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Citric acid
C6H8O7 192.123 g/mol
|
0.3 G_PER_L |
CHEBI:30769
citric acid
|
Properties: Simple component, Defined component, Organic compound |
| 7 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.079 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Co(NO3)2・6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.049 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
15.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 14 | Ferric ammonium citrate | 12 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 15 |
2-Oxoglutarate
C5H4O5 144.082 g/mol
|
14.6 G_PER_L |
CHEBI:16810
2-oxoglutarate(2-)
|
Role: Growth factor |
| 16 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.625 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.125 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Simple component, Organic compound |
| 18 | Bacto peptone (BD--Difco) | 10 G_PER_L | Properties: Undefined component | |
| 19 |
L--Cysteine
C3H7NO2S 121.161 g/mol
|
100 G_PER_L |
CHEBI:17561
L-cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 20 |
Glycine
C2H5NO2 75.067 g/mol
|
100 G_PER_L |
CHEBI:15428
glycine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 21 |
L--Glutamic acid
C5H9NO4 147.13 g/mol
|
100 G_PER_L |
CHEBI:16015
L-glutamic acid
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 22 |
L--Alanine
C3H7NO2 89.094 g/mol
|
100 G_PER_L |
CHEBI:16977
L-alanine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 23 |
L--Arginine
C6H14N4O2 174.204 g/mol
|
100 G_PER_L |
CHEBI:16467
L-arginine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 24 |
L--Aspartic acid
C4H7NO4 133.103 g/mol
|
100 G_PER_L |
CHEBI:17053
L-aspartic acid
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 25 |
L--Glutamine
C5H10N2O3 146.146 g/mol
|
100 G_PER_L |
CHEBI:18050
L-glutamine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 26 |
L--Isoleucine
C6H13NO2 131.175 g/mol
|
100 G_PER_L |
CHEBI:17191
L-isoleucine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 27 |
L--Leucine
C6H13NO2 131.175 g/mol
|
100 G_PER_L |
CHEBI:15603
L-leucine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 28 |
L--Lysine
C6H14N2O2 146.19 g/mol
|
100 G_PER_L |
CHEBI:18019
L-lysine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 29 |
L--Phenylalanine
C9H11NO2 165.192 g/mol
|
100 G_PER_L |
CHEBI:17295
L-phenylalanine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 30 |
L--Proline
C5H9NO2 115.132 g/mol
|
100 G_PER_L |
CHEBI:17203
L-proline
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 31 |
L--Serine
C3H7NO3 105.093 g/mol
|
100 G_PER_L |
CHEBI:17115
L-serine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 32 |
L--Threonine
C4H9NO3 119.12 g/mol
|
100 G_PER_L |
CHEBI:16857
L-threonine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 33 |
L--Tryptophan
C11H12N2O2 204.229 g/mol
|
100 G_PER_L |
CHEBI:16828
L-tryptophan
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 34 |
L--Tyrosine
C9H11NO3 181.191 g/mol
|
100 G_PER_L |
CHEBI:17895
L-tyrosine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 35 |
L--Valine
C5H11NO2 117.148 g/mol
|
100 G_PER_L |
CHEBI:16414
L-valine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 36 |
L--Asparagine
C4H8N2O3 132.119 g/mol
|
100 G_PER_L |
CHEBI:17196
L-asparagine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 37 |
L--Histidine
C6H9N3O2 155.157 g/mol
|
100 G_PER_L |
CHEBI:15971
L-histidine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 38 |
L--Methionine
C5H11NO2S 149.215 g/mol
|
100 G_PER_L |
CHEBI:16643
L-methionine
|
Role: Nitrogen source |
| 39 | 10 mM Potassium phosphate buffer (pH 7.2) | 100 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound, Complex component | |
| 40 |
Biotin
C10H16N2O3S 244.316 g/mol
|
10 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 41 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
100 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 42 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
100 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 43 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 44 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
100 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 45 |
Folic acid
C19H19N7O6 441.404 g/mol
|
100 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 46 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
10 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 47 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
100 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 48 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
100 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 49 |
Thiamine pyrophosphate
C12H19N4O7P2S 425.32 g/mol
|
100 G_PER_L |
CHEBI:9532
thiamine(1+) diphosphate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 50 | β-NAD | 100 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 51 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
100 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 52 |
L--Ascorbic acid
C6H8O6 176.124 g/mol
|
100 G_PER_L |
CHEBI:29073
L-ascorbic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 53 | Vitamin B12 | 100 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution 1 (see below)
Dose: 20 G_PER_L
Solution 2 (see below)
Dose: 3 G_PER_L
Solution 3 (see below)
Dose: 2 G_PER_L
Solution 4 (see below)
Dose: 2.5 G_PER_L
Mixed solution
Dose: 30 G_PER_L
See mediadive_5182_Mixed_solution.yaml for composition
0.25 M EDTA・2Na solution (pH 8.0)
Dose: 0.6 G_PER_L
Properties: Defined component, Organic compound, Solution
Trace metal solution (see below)
Dose: 50 G_PER_L
Solution 5 (see below)
Dose: 0.5 G_PER_L
Solution 6 (see below)
Dose: 10 G_PER_L
Vitamin solution A (see below)
Dose: 0.25 G_PER_L
Vitamin solution B (see below)
Dose: 0.25 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.058140Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1173 |
| 2026-01-28T19:48:29.335578Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:48.724838+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 11 solution(s) from ingredients |
| 2026-03-13T03:09:19.843409Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 9 duplicate ingredient(s) |
| 2026-03-13T06:19:50.721105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007698 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.123610+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CTM Medium" to "ctm_medium" for consistent matching |
| 2026-05-16T09:03:08.161107+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.049817+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:29.988652+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:21.672795+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.117237+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:17.189730+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 6 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |