halolactibacillus_9g_medium (CultureMech:007699)

Overview

External ontology

TOGO:M1174 — Halolactibacillus 9G Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M1174 Original source: JCM - JCM_M1101 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1101

Composition

flowchart LR
    CultureMech_007699["halolactibacillus_9g_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007699 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007699 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_007699 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007699 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2HPO4["K2HPO4"]
    CultureMech_007699 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_007699 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007699 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_007699 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_N2["N2"]
    CultureMech_007699 --> ing8_N2
    CHEBI_17997["CHEBI:17997"]
    ing8_N2 -.-> CHEBI_17997
    ing9_NaHCO3["NaHCO3"]
    CultureMech_007699 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2CO3["Na2CO3"]
    CultureMech_007699 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377

Ingredients (11)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	980.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 680.0, 300.0]
2	Yeast extract	0.1 G_PER_L		Role: Nutrient source; Properties: Undefined component
3	NaCl Cl.Na 58.443 g/mol	10 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4	NH4Cl Cl.H4N 53.492 g/mol	1 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
5	K2HPO4 HO4P.2K 174.174 g/mol	0.2 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
6	Resazurin C12H7NO4 229.191 g/mol	0.5 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
7	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.1 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8	KCl Cl.K 74.551 g/mol	0.2 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
9	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
10	NaHCO3 CHO3.Na 84.006 g/mol	24 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
11	Na2CO3 CO3.2Na 105.988 g/mol	2.76 G_PER_L	CHEBI:29377 sodium carbonate	Role: Buffer; Properties: Defined component, Simple component, Inorganic compound

Solutions

Trace vitamins (see Medium [M190])

Dose: 2 G_PER_L

Cross-reference to Medium M190

Trace minerals solution (see Medium [M221])

Dose: 1 G_PER_L

Cross-reference to Medium M221

1.0 M Glucose solution (autoclaved)

Dose: 20 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

Alkaline solution (see below)

Dose: 300 G_PER_L

5% Na2S・9H2O solution

Dose: 8 G_PER_L

Role: Reducing agent; Properties: Inorganic compound, Solution, Defined component

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.065467Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1174
2026-01-28T19:48:34.948182Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: N2; Added default concentration for ingredient: N2
2026-03-09T08:04:58.024362+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 5 solution(s) from ingredients
2026-03-13T03:09:29.656944Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 2 duplicate ingredient(s)
2026-03-13T06:19:50.736749Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007699
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:39.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.140369+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Halolactibacillus 9G Medium" to "halolactibacillus_9g_medium" for consistent matching
2026-05-16T09:03:08.179665+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:31.068294+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.129974+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.