anaerobic_natural_seawater_medium

CultureMech:007700 bacterial COMPLEX LIQUID
Original name: Anaerobic Natural Seawater Medium

Overview

External ontology
TOGO:M1175 — Anaerobic Natural Seawater Medium
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M1175 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, resazurin, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. After autoclaving and cooling, the source adds 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions.

Composition

flowchart LR
    CultureMech_007700["anaerobic_natural_seawater_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_007700 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007700 --> ing1_Yeast_extract
    ing2_Resazurin["Resazurin"]
    CultureMech_007700 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Natural_seawater__filtrated_["Natural seawater (filtrated)"]
    CultureMech_007700 --> ing3_Natural_seawater__filtrated_
    ing4_Distilled_water["Distilled water"]
    CultureMech_007700 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_N2["N2"]
    CultureMech_007700 --> ing5_N2
    CHEBI_17997["CHEBI:17997"]
    ing5_N2 -.-> CHEBI_17997

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Tryptone 5.0 G_PER_L JCM Medium 1102 lists 5.0 g/L Tryptone.
2 Yeast extract 1.0 G_PER_L JCM Medium 1102 lists 1.0 g/L Yeast extract.
3 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
 JCM Medium 1102 lists 0.5 mg/L Resazurin.
4 Natural seawater (filtrated) 750.0 ML_PER_L JCM Medium 1102 lists 750.0 ml/L Natural seawater (filtrated).
5 Distilled water
H2O 18.015 g/mol
250.0 ML_PER_L CHEBI:15377
water
 JCM Medium 1102 lists 250.0 ml/L Distilled water.
6 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 JCM Medium 1102 cools the boiled base, distributes the medium, and stores reducing stocks under an N2 gas atmosphere.

Preparation

  1. Mix tryptone, yeast extract, resazurin, natural seawater, and distilled water.
  2. Adjust to pH 7.0.
  3. Bring the medium to a boil and cool under an N2 gas atmosphere.
  4. Distribute the medium in culture vessels under N2 and seal with butyl rubber stoppers.
  5. Autoclave at 121 degrees C for 15 min.
  6. After cooling, aseptically and anaerobically add 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions autoclaved and stored under N2.

Solutions

5% L-Cysteine HCl H2O solution

Dose: 6.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl H2O 5.0 PERCENT_W_V 5% L-Cysteine HCl H2O solution is represented from the stock label as 5.0% w/v L-Cysteine HCl H2O.
JCM Medium 1102 adds 6.0 ml/L 5% L-Cysteine HCl H2O solution.

5% Na2S x 9H2O solution

Dose: 6.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S x 9H2O 5.0 PERCENT_W_V 5% Na2S x 9H2O solution is represented from the stock label as 5.0% w/v Na2S x 9H2O.
JCM Medium 1102 adds 6.0 ml/L 5% Na2S x 9H2O solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.067579Z togo-import Imported from TOGO Medium Source: JCM, ID: M1175
2026-01-28T19:48:21.967972Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:36.672478+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:50.743594Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007700
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.147547+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Anaerobic Natural Seawater Medium" to "anaerobic_natural_seawater_medium" for consistent matching
2026-05-16T09:03:24.362381+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:48.928267+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m1175_anaerobic_natural_seawater_score15.py RESOLVED_TOGO_M1175_ANAEROBIC_NATURAL_SEAWATER TOGO M1175 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, resazurin, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. After autoclaving and cooling, the source adds 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions.