Overview
- External ontology
- TOGO:M1177 — Anaerobic Natural Seawater Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M1177 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. For strain JCM 33129, the source omits resazurin and the L-Cysteine HCl H2O and Na2S x 9H2O reducing agents. JCM 33129 variant omitting resazurin and reducing agents.
Composition
flowchart LR
CultureMech_007702["anaerobic_natural_seawater_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_007702 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_007702 --> ing1_Yeast_extract
ing2_Natural_seawater__filtrated_["Natural seawater (filtrated)"]
CultureMech_007702 --> ing2_Natural_seawater__filtrated_
ing3_Distilled_water["Distilled water"]
CultureMech_007702 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_N2["N2"]
CultureMech_007702 --> ing4_N2
CHEBI_17997["CHEBI:17997"]
ing4_N2 -.-> CHEBI_17997
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 5.0 G_PER_L | JCM Medium 1102 lists 5.0 g/L Tryptone. | |
| 2 | Yeast extract | 1.0 G_PER_L | JCM Medium 1102 lists 1.0 g/L Yeast extract. | |
| 3 | Natural seawater (filtrated) | 750.0 ML_PER_L | JCM Medium 1102 lists 750.0 ml/L Natural seawater (filtrated). | |
| 4 |
Distilled water
H2O 18.015 g/mol
|
250.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1102 lists 250.0 ml/L Distilled water. |
| 5 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1102 cools the boiled base, distributes the medium, and stores reducing stocks under an N2 gas atmosphere. |
Preparation
- Mix tryptone, yeast extract, natural seawater, and distilled water.
- Adjust to pH 7.0.
- Bring the medium to a boil and cool under an N2 gas atmosphere.
- Distribute the medium in culture vessels under N2 and seal with butyl rubber stoppers.
- Autoclave at 121 degrees C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.070137Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1177 |
| 2026-01-28T19:48:25.024504Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:19:50.754000Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007702 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.158221+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerobic Natural Seawater Medium" to "anaerobic_natural_seawater_medium" for consistent matching |
| 2026-05-16T09:03:08.193555+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.083014+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1176_m1178_score15.py | RESOLVED_TOGO_M1176_M1178_SCORE15 | TOGO M1177 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. For strain JCM 33129, the source omits resazurin and the L-Cysteine HCl H2O and Na2S x 9H2O reducing agents. JCM 33129 variant omitting resazurin and reducing agents. |