Overview
- External ontology
- TOGO:M1178 — Anaerobic Natural Seawater Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M1178 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, resazurin, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. After autoclaving and cooling, the source adds 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions. For strain JCM 33131, the source supplements 10.0 ml/L Trace vitamins from JCM Medium 197. JCM 33131 variant supplemented with trace vitamins.
Composition
flowchart LR
CultureMech_007703["anaerobic_natural_seawater_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_007703 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_007703 --> ing1_Yeast_extract
ing2_Resazurin["Resazurin"]
CultureMech_007703 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Natural_seawater__filtrated_["Natural seawater (filtrated)"]
CultureMech_007703 --> ing3_Natural_seawater__filtrated_
ing4_Distilled_water["Distilled water"]
CultureMech_007703 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_N2["N2"]
CultureMech_007703 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 5.0 G_PER_L | JCM Medium 1102 lists 5.0 g/L Tryptone. | |
| 2 | Yeast extract | 1.0 G_PER_L | JCM Medium 1102 lists 1.0 g/L Yeast extract. | |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
JCM Medium 1102 lists 0.5 mg/L Resazurin. |
| 4 | Natural seawater (filtrated) | 750.0 ML_PER_L | JCM Medium 1102 lists 750.0 ml/L Natural seawater (filtrated). | |
| 5 |
Distilled water
H2O 18.015 g/mol
|
250.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1102 lists 250.0 ml/L Distilled water. |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1102 cools the boiled base, distributes the medium, and stores reducing stocks under an N2 gas atmosphere. |
Preparation
- Mix tryptone, yeast extract, resazurin, natural seawater, and distilled water.
- Adjust to pH 7.0.
- Bring the medium to a boil and cool under an N2 gas atmosphere.
- Distribute the medium in culture vessels under N2 and seal with butyl rubber stoppers.
- Autoclave at 121 degrees C for 15 min.
- After cooling, aseptically and anaerobically add 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions autoclaved and stored under N2.
- Supplement the JCM 33131 variant with 10.0 ml/L Trace vitamins from JCM Medium 197.
Solutions
5% L-Cysteine HCl H2O solution
Dose: 6.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine HCl H2O | 5.0 PERCENT_W_V | 5% L-Cysteine HCl H2O solution is represented from the stock label as 5.0% w/v L-Cysteine HCl H2O. |
JCM Medium 1102 adds 6.0 ml/L 5% L-Cysteine HCl H2O solution.
5% Na2S x 9H2O solution
Dose: 6.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 5.0 PERCENT_W_V | 5% Na2S x 9H2O solution is represented from the stock label as 5.0% w/v Na2S x 9H2O. |
JCM Medium 1102 adds 6.0 ml/L 5% Na2S x 9H2O solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | JCM Medium 197 lists 1.0 L Distilled water. |
JCM Medium 1102 supplements the JCM 33131 variant with 10.0 ml/L Trace vitamins.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.071048Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1178 |
| 2026-01-28T19:48:41.577134Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:10.140781+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:50.758993Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007703 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.163245+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerobic Natural Seawater Medium" to "anaerobic_natural_seawater_medium" for consistent matching |
| 2026-05-16T09:03:08.199338+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.088987+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1176_m1178_score15.py | RESOLVED_TOGO_M1176_M1178_SCORE15 | TOGO M1178 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, resazurin, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. After autoclaving and cooling, the source adds 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions. For strain JCM 33131, the source supplements 10.0 ml/L Trace vitamins from JCM Medium 197. JCM 33131 variant supplemented with trace vitamins. |