liquid_basal_medium_for_methanosarcina

CultureMech:007710 archaea COMPLEX LIQUID
Original name: Liquid Basal Medium For Methanosarcina

Overview

External ontology
TOGO:M1184 — Liquid Basal Medium For Methanosarcina
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1184 Original source: JCM - JCM_M1107 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1107

Composition

flowchart LR
    CultureMech_007710["liquid_basal_medium_for_methanosarcina"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007710 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007710 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007710 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007710 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007710 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_007710 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007710 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_MOPS["MOPS"]
    CultureMech_007710 --> ing7_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing7_MOPS -.-> CHEBI_39074
    ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007710 --> ing8_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing8_Carbon_dioxide_gas -.-> CHEBI_16526
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007710 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997
    ing10_N2["N2"]
    CultureMech_007710 --> ing10_N2
    ing10_N2 -.-> CHEBI_17997
    ing11_Na2MoO4_2H2O["Na2MoO4・2H2O"]
    CultureMech_007710 --> ing11_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_2H2O -.-> CHEBI_75213
    ing12_H3BO3["H3BO3"]
    CultureMech_007710 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_007710 --> ing13_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_7H2O -.-> CHEBI_75836
    ing14_CoCl2_6H2O["CoCl2・6H2O"]
    CultureMech_007710 --> ing14_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing14_CoCl2_6H2O -.-> CHEBI_35696
    ing15_NiCl2_6H2O["NiCl2・6H2O"]
    CultureMech_007710 --> ing15_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_6H2O -.-> CHEBI_34887
    ing16_ZnSO4_7H2O["ZnSO4・7H2O"]
    CultureMech_007710 --> ing16_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_7H2O -.-> CHEBI_32312
    ing17_CuSO4_5H2O["CuSO4・5H2O"]
    CultureMech_007710 --> ing17_CuSO4_5H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_5H2O -.-> CHEBI_31440
    ing18_Na2SeO3_5H2O["Na2SeO3・5H2O"]
    CultureMech_007710 --> ing18_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_5H2O -.-> CHEBI_131361
    ing19_Na2WO4_2H2O["Na2WO4・2H2O"]
    CultureMech_007710 --> ing19_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_2H2O -.-> CHEBI_63939
    ing20_MnSO4_5H2O["MnSO4・5H2O"]
    CultureMech_007710 --> ing20_MnSO4_5H2O
    CHEBI_131524["CHEBI:131524"]
    ing20_MnSO4_5H2O -.-> CHEBI_131524
    ing21_Nitrilotriacetic_acid_trisodium_salt_monohydrate["Nitrilotriacetic acid trisodium salt monohydrate"]
    CultureMech_007710 --> ing21_Nitrilotriacetic_acid_trisodium_salt_monohydrate

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
931.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 930.0, 1.0]
2 NaCl
Cl.Na 58.443 g/mol
0.4 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.4 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 MOPS 4.2 G_PER_L CHEBI:39074
MOPS
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
9 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
10 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
12 Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
5.55 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 CoCl2・6H2O
2Cl.Co 129.839 g/mol
1 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 NiCl2・6H2O
Cl2Ni 129.599 g/mol
3 G_PER_L CHEBI:34887
nickel dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19 Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
0.3 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20 Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 MnSO4・5H2O
5H2O.Mn.O4S 241.076 g/mol
1 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 Nitrilotriacetic acid trisodium salt monohydrate 11.1 G_PER_L Role: Mineral source; Properties: Organic compound, Defined component, Simple component

Solutions

Trace element solution (see below)

Dose: 1 G_PER_L

8% NaHCO3 solution*

Dose: 50 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

50% (v/v) Methanol solution

Dose: 10 G_PER_L

Role: Solvating media; Properties: Solution, Organic compound, Defined component

Vitamin solution (see Medium [M991])

Dose: 10 G_PER_L

Cross-reference to Medium M991

5% Na2S・9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

5% L--Cysteine・HCl・H2O solution

Dose: 10 G_PER_L

Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.082690Z togo-import Imported from TOGO Medium Source: JCM, ID: M1184
2026-01-28T19:48:29.988569Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2
2026-03-09T08:04:49.831313+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T03:09:21.009394Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:50.815895Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007710
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.220780+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Liquid Basal Medium For Methanosarcina" to "liquid_basal_medium_for_methanosarcina" for consistent matching
2026-05-16T09:03:08.251620+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.141276+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.145703+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.