methanosarcina_subterranea_medium

CultureMech:007713 archaea COMPLEX LIQUID
Original name: Methanosarcina Subterranea Medium

Overview

External ontology
TOGO:M1187 — Methanosarcina Subterranea Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1187 Original source: JCM - JCM_M1110 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1110

Composition

flowchart LR
    CultureMech_007713["methanosarcina_subterranea_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007713 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007713 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007713 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007713 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_007713 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_007713 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_K2HPO4["K2HPO4"]
    CultureMech_007713 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Resazurin["Resazurin"]
    CultureMech_007713 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_007713 --> ing8_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_9H2O -.-> CHEBI_76209
    ing9_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_007713 --> ing9_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_7H2O -.-> CHEBI_75836
    ing10_NaHCO3["NaHCO3"]
    CultureMech_007713 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Methanol["Methanol"]
    CultureMech_007713 --> ing11_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing11_Methanol -.-> CHEBI_17790
    ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007713 --> ing12_Yeast_extract__BD_Difco_
    ing13_Casitone__BD_Difco_["Casitone (BD-Difco)"]
    CultureMech_007713 --> ing13_Casitone__BD_Difco_
    ing14_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007713 --> ing14_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing14_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007713 --> ing15_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing15_Carbon_dioxide_gas -.-> CHEBI_16526
    ing16_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007713 --> ing16_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing16_Nitrogen_gas -.-> CHEBI_17997

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
980 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3 NaCl
Cl.Na 58.443 g/mol
6 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.227 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
6 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
7 K2HPO4
HO4P.2K 174.174 g/mol
0.348 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
8 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
9 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.3 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
10 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 NaHCO3
CHO3.Na 84.006 g/mol
0.85 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
12 Methanol
CH4O 32.042 g/mol
10 G_PER_L CHEBI:17790
methanol
 Role: Solvating media; Properties: Organic compound, Simple component, Defined component
13 Yeast extract (BD-Difco) 2 G_PER_L Properties: Undefined component
14 Casitone (BD-Difco) 2 G_PER_L Properties: Undefined component
15 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
16 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
17 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.088544Z togo-import Imported from TOGO Medium Source: JCM, ID: M1187
2026-01-28T19:48:37.619703Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:02.802817+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:50.839480Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007713
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.245021+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanosarcina Subterranea Medium" to "methanosarcina_subterranea_medium" for consistent matching
2026-05-16T09:03:08.274552+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.164581+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.758064+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.155866+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.