anaerobic_mineral_medium_with_yeast_extract (CultureMech:007716)

Overview

External ontology

TOGO:M1190 — Anaerobic Mineral Medium With Yeast Extract

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M1190 Original source: JCM - JCM_M1113 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1113

Composition

flowchart LR
    CultureMech_007716["anaerobic_mineral_medium_with_yeast_extract"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007716 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007716 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007716 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_007716 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007716 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_007716 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_007716 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_KCl["KCl"]
    CultureMech_007716 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_CaSO4_2H2O["CaSO4·2H2O"]
    CultureMech_007716 --> ing8_CaSO4_2H2O
    CHEBI_32583["CHEBI:32583"]
    ing8_CaSO4_2H2O -.-> CHEBI_32583
    ing9_N2["N2"]
    CultureMech_007716 --> ing9_N2
    CHEBI_17997["CHEBI:17997"]
    ing9_N2 -.-> CHEBI_17997

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	MgSO4·7H2O 7H2O.Mg.O4S 246.473 g/mol	0.1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3	Yeast extract	0.1 G_PER_L		Role: Nutrient source; Properties: Undefined component
4	NaCl Cl.Na 58.443 g/mol	2.5 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5	NH4Cl Cl.H4N 53.492 g/mol	1 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
6	K2HPO4 HO4P.2K 174.174 g/mol	0.3 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
7	Resazurin C12H7NO4 229.191 g/mol	0.5 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
8	KCl Cl.K 74.551 g/mol	0.3 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
9	CaSO4·2H2O Ca.2H2O.O4S 172.171 g/mol	12.5 G_PER_L	CHEBI:32583 calcium sulfate dihydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
10	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: Gas

Solutions

Fatty acid mixture (see Medium [M258])

Dose: 10 G_PER_L

Cross-reference to Medium M258

Trace vitamins (see Medium [M190])

Dose: 2 G_PER_L

Cross-reference to Medium M190

Trace minerals solution (see Medium [M221])

Dose: 1 G_PER_L

Cross-reference to Medium M221

5% Na2S・9H2O solution (pH 7.0)

Dose: 8 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

1.0 M Sodium thiosulfate solution

Dose: 10 G_PER_L

Properties: Defined component, Inorganic compound, Solution

10% Peptone solution

Dose: 30 G_PER_L

Properties: Undefined component

Trace vitamins (see Medium [M190])

Dose: 2 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.093334Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1190
2026-01-28T19:48:37.086407Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: N2
2026-03-09T08:05:01.902703+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 7 solution(s) from ingredients
2026-03-13T06:19:50.858803Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007716
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:44.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.265289+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Anaerobic Mineral Medium With Yeast Extract" to "anaerobic_mineral_medium_with_yeast_extract" for consistent matching
2026-05-16T09:03:24.354240+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:48.919937+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:36.249330+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:53.179842+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.