modified_sw_25_medium (CultureMech:007718)

Overview

External ontology

TOGO:M1192 — Modified SW-25 Medium

Applications

Microbial cultivation

pH

7.5

Notes

TOGO M1192 records JCM_M1114-2 as a solid-agar Modified SW-25 Medium variant. JCM Medium 1114 / MediaDive J1114 supplies the complete SW-25 solution composition, 1.0 g/L each yeast extract and Casamino acids, pH 7.5, and a 20.0 g/L agar supplement for solid medium.

Composition

flowchart LR
    CultureMech_007718["modified_sw_25_medium"]
    ing0_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_007718 --> ing0_Casamino_acids__BD_Difco_
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007718 --> ing1_Yeast_extract
    ing2_Agar["Agar"]
    CultureMech_007718 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509

Ingredients (3)

#	Ingredient	Concentration	Ontology	Notes
1	Casamino acids (BD-Difco)	1.0 G_PER_L		JCM Medium 1114 lists 1.0 g/L Casamino acids with BD-Difco attribute.
2	Yeast extract	1.0 G_PER_L		JCM Medium 1114 / MediaDive J1114 lists 1.0 g/L Yeast extract.
3	Agar	20.0 G_PER_L	CHEBI:2509 agar	JCM Medium 1114 / MediaDive J1114 lists 20.0 g/L Agar.

Preparation

Mix SW-25 solution, Casamino acids, yeast extract, and agar.
Adjust to pH 7.5.
Autoclave the solid medium.

Solutions

SW-25 solution

Dose: 1000.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaCl	195.0 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 195.0 g/L NaCl.
MgCl2 x 6H2O	32.5 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 32.5 g/L MgCl2 x 6H2O.
MgSO4 x 7H2O	51.0 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 51.0 g/L MgSO4 x 7H2O.
KCl	5.0 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 5.0 g/L KCl.
NaBr	0.6 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 0.6 g/L NaBr.
NaHCO3	0.16 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 0.16 g/L NaHCO3.
CaCl2 x 2H2O	0.8 G_PER_L	JCM Medium 1114 / MediaDive J1114 lists 0.8 g/L CaCl2 x 2H2O.
Distilled water	1.0 L	JCM Medium 1114 / MediaDive J1114 lists 1.0 L Distilled water.

JCM Medium 1114 adds 1000 ml SW-25 solution to the main recipe.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.096978Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1192
2026-01-28T19:48:23.086579Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:38.338060+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:19:50.871039Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007718
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:14.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.278554+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified SW-25 Medium" to "modified_sw_25_medium" for consistent matching
2026-05-16T09:03:08.301423+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:31.189101+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00	repair_togo_m1192_m1198_score15.py	RESOLVED_TOGO_M1192_M1198_SCORE15	TOGO M1192 records JCM_M1114-2 as a solid-agar Modified SW-25 Medium variant. JCM Medium 1114 / MediaDive J1114 supplies the complete SW-25 solution composition, 1.0 g/L each yeast extract and Casamino acids, pH 7.5, and a 20.0 g/L agar supplement for solid medium.