Overview
- External ontology
- TOGO:M1198 — Ethane-C Medium
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- TOGO M1198 records JCM_M1120 as Ethane-C Medium. MediaDive J1120 supplies the complete main solution at pH 8.0, the Vitamin mixture, Metal mixture, and Artificial seawater compositions cross-referenced from TOGO M1196, 110 degrees C autoclaving for 5 min, overnight standing after sterilization, and 10-50% v/v ethane in the gas phase.
Composition
flowchart LR
CultureMech_007723["ethane_c_medium"]
ing0_NH4NO3["NH4NO3"]
CultureMech_007723 --> ing0_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing0_NH4NO3 -.-> CHEBI_63038
ing1_KH2PO4["KH2PO4"]
CultureMech_007723 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_007723 --> ing2_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing2_Fe_III__EDTA -.-> CHEBI_30729
ing3_NaOH["NaOH"]
CultureMech_007723 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
ing4_Ethane["Ethane"]
CultureMech_007723 --> ing4_Ethane
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.0996016 G_PER_L |
CHEBI:63038
ammonium nitrate
|
JCM Medium 1120 lists 0.1 g NH4NO3 in the 1004 ml main solution; MediaDive normalizes this to 0.0996016 g/L. |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.00996016 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 1120 lists 0.01 g KH2PO4 in the 1004 ml main solution; MediaDive normalizes this to 0.00996016 g/L. |
| 3 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
0.00249004 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
JCM Medium 1120 lists 2.5 mg Fe(III)-EDTA in the 1004 ml main solution; MediaDive normalizes this to 0.00249004 g/L. |
| 4 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
JCM Medium 1120 adjusts the main solution to pH 8.0 with NaOH. |
| 5 | Ethane | 10-50 PERCENT_V_V | JCM Medium 1120 adds ethane to 10-50% of the gas phase by volume. |
Preparation
- Mix NH4NO3, KH2PO4, Fe(III)-EDTA, Vitamin mixture, Metal mixture, and Artificial seawater.
- Adjust to pH 8.0 with NaOH.
- Distribute 20-50 ml aliquots into 120 ml serum bottles and seal with butyl rubber stoppers.
- Autoclave at 110 degrees C for 5 min.
- Allow the autoclaved medium to stand overnight, then add ethane to 10-50% of the gas phase by volume.
Solutions
Vitamin mixture
Dose: 2.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 1.1 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 1.1 mg/L Vitamin B12. |
| Biotin | 1.0 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 1.0 mg/L Biotin. |
| Thiamine HCl | 200.0 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 200.0 mg/L Thiamine HCl. |
| Distilled water | 1.0 L | JCM Medium 1120 / MediaDive J1120 lists 1.0 L Distilled water. |
JCM Medium 1120 adds 2.5 ml Vitamin mixture to the main solution.
Metal mixture
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA x 2Na | 0.372 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.372 g/L EDTA x 2Na. |
| CuSO4 x 5H2O | 0.25 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.25 mg/L CuSO4 x 5H2O. |
| ZnSO4 x 7H2O | 5.75 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 5.75 mg/L ZnSO4 x 7H2O. |
| MnCl2 x 6H2O | 4.55 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 4.55 mg/L MnCl2 x 6H2O. |
| CoCl2 x 6H2O | 0.6 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.6 mg/L CoCl2 x 6H2O. |
| (NH4)6Mo7O24 x 4H2O | 0.27 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.27 mg/L (NH4)6Mo7O24 x 4H2O. |
| Distilled water | 1.0 L | JCM Medium 1120 / MediaDive J1120 lists 1.0 L Distilled water. |
JCM Medium 1120 adds 1.0 ml Metal mixture to the main solution.
Artificial seawater
Dose: 1000.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 26.3 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 26.3 g/L NaCl. |
| CaCl2 x 2H2O | 1.54 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 1.54 g/L CaCl2 x 2H2O. |
| KBr | 0.1 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.1 g/L KBr. |
| KF | 0.004 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.004 g/L KF. |
| KCl | 0.7 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.7 g/L KCl. |
| H3BO3 | 30.0 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 30.0 mg/L H3BO3. |
| MgSO4 x 7H2O | 7.0 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 7.0 g/L MgSO4 x 7H2O. |
| KHCO3 | 0.24 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 0.24 g/L KHCO3. |
| SrCl2 x 6H2O | 17.0 MG_PER_L | JCM Medium 1120 / MediaDive J1120 lists 17.0 mg/L SrCl2 x 6H2O. |
| MgCl2 x 6H2O | 5.2 G_PER_L | JCM Medium 1120 / MediaDive J1120 lists 5.2 g/L MgCl2 x 6H2O. |
| Distilled water | 1.0 L | JCM Medium 1120 / MediaDive J1120 lists 1.0 L Distilled water. |
JCM Medium 1120 adds 1000 ml Artificial seawater to the main solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.107681Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1198 |
| 2026-01-28T19:48:42.016063Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:05:10.820976+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:50.918436Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007723 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.326556+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ethane-C Medium" to "ethane_c_medium" for consistent matching |
| 2026-05-16T09:03:08.333299+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.221741+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.179082+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1192_m1198_score15.py | RESOLVED_TOGO_M1192_M1198_SCORE15 | TOGO M1198 records JCM_M1120 as Ethane-C Medium. MediaDive J1120 supplies the complete main solution at pH 8.0, the Vitamin mixture, Metal mixture, and Artificial seawater compositions cross-referenced from TOGO M1196, 110 degrees C autoclaving for 5 min, overnight standing after sterilization, and 10-50% v/v ethane in the gas phase. |