methanol_utilizing_bacteria_medium_e (CultureMech:007725)

Overview

External ontology

TOGO:M119 — Methanol-Utilizing Bacteria Medium E

Applications

Microbial cultivation

Notes

TOGO M119 mirrors JCM Medium 127: combine 1.0 L Methanol-Utilizing Bacteria Medium B with 30.0 g NaCl and 10.0 micrograms Vitamin B12.

Composition

flowchart LR
    CultureMech_007725["methanol_utilizing_bacteria_medium_e"]
    ing0_NaCl["NaCl"]
    CultureMech_007725 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Vitamin_B12["Vitamin B12"]
    CultureMech_007725 --> ing1_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing1_Vitamin_B12 -.-> CHEBI_176843

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	NaCl Cl.Na 58.443 g/mol	30.0 G_PER_L	CHEBI:26710 sodium chloride	JCM Medium 127 adds 30.0 g NaCl per liter of Medium B.
2	Vitamin B12	10.0 MICROG_PER_L	CHEBI:176843 vitamin B12	JCM Medium 127 adds 10.0 micrograms Vitamin B12 per liter of Medium B.

Preparation

Combine 1.0 L Methanol-utilizing bacteria medium B with 30.0 g NaCl and 10.0 micrograms Vitamin B12.
Autoclave at 121 C for 15 min.

Solutions

Methanol-utilizing bacteria medium B

Dose: 1000 ML_PER_L

CultureMech:003144

JCM Medium 127 lists 1.0 L Methanol-utilizing bacteria medium B.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.092461Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M119
2026-01-28T19:48:26.350829Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:43.744803+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:19:50.932553Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007725
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:21.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.341309+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Methanol-Utilizing Bacteria Medium E" to "methanol_utilizing_bacteria_medium_e" for consistent matching
2026-05-16T09:03:08.350293+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:31.239203+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-06T00:00:00-07:00	repair_methanol_utilizing_score40.py	RESOLVED_METHANOL_UTILIZING_SCORE40_GRAPH	TOGO M119 mirrors JCM Medium 127: combine 1.0 L Methanol-Utilizing Bacteria Medium B with 30.0 g NaCl and 10.0 micrograms Vitamin B12.