Overview
- External ontology
- TOGO:M11 — Lactic Acid Bacteria Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M11 Original source: JCM - JCM_M18 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=18
Composition
flowchart LR
CultureMech_007726["lactic_acid_bacteria_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_007726 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sodium_acetate["Sodium acetate"]
CultureMech_007726 --> ing1_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing1_Sodium_acetate -.-> CHEBI_32954
ing2_Glucose["Glucose"]
CultureMech_007726 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_007726 --> ing3_Trypticase_peptone__BD_BBL_
ing4_Yeast_autolysate["Yeast autolysate"]
CultureMech_007726 --> ing4_Yeast_autolysate
ing5_KH2PO4["KH2PO4"]
CultureMech_007726 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_K2HPO4["K2HPO4"]
CultureMech_007726 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007726 --> ing7_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_7H2O -.-> CHEBI_31795
ing8_NaCl["NaCl"]
CultureMech_007726 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007726 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_007726 --> ing10_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing10_MnSO4_xH2O -.-> CHEBI_86360
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
201.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 1.0, 100.0, 100.0] |
| 2 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
12 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 | Trypticase peptone (BD-BBL) | 10 G_PER_L | Properties: Undefined component | |
| 5 | Yeast autolysate | 5 G_PER_L | Properties: Undefined component | |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
0.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
Solution A (see below)
Dose: 5 G_PER_L
Solution B (see below)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.915359Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M11 |
| 2026-01-28T19:48:28.059408Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:46.454660+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:17.037895Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:50.938748Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007726 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.347566+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lactic Acid Bacteria Broth" to "lactic_acid_bacteria_broth" for consistent matching |
| 2026-05-16T09:03:08.357244+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.246698+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:21.832514+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.186157+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |