Overview
- External ontology
- TOGO:M1206 — Sulfate-Free Metako Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1206 Original source: JCM - JCM_M1126-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1126
Composition
flowchart LR
CultureMech_007733["sulfate_free_metako_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007733 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007733 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007733 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_007733 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_007733 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_007733 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007733 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_007733 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_007733 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_007733 --> ing9_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing9_SrCl2_6H2O -.-> CHEBI_36385
ing10_NaF["NaF"]
CultureMech_007733 --> ing10_NaF
CHEBI_28741["CHEBI:28741"]
ing10_NaF -.-> CHEBI_28741
ing11_KBr["KBr"]
CultureMech_007733 --> ing11_KBr
CHEBI_32030["CHEBI:32030"]
ing11_KBr -.-> CHEBI_32030
ing12_LiCl["LiCl"]
CultureMech_007733 --> ing12_LiCl
CHEBI_48607["CHEBI:48607"]
ing12_LiCl -.-> CHEBI_48607
ing13_Na2B4O7_10H2O["Na2B4O7・10H2O"]
CultureMech_007733 --> ing13_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing13_Na2B4O7_10H2O -.-> CHEBI_131366
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007733 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_007733 --> ing15_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen_gas -.-> CHEBI_17997
ing16_yeast_extract["yeast extract"]
CultureMech_007733 --> ing16_yeast_extract
ing17_sodium_lactate["sodium lactate"]
CultureMech_007733 --> ing17_sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing17_sodium_lactate -.-> CHEBI_75228
ing18_N2["N2"]
CultureMech_007733 --> ing18_N2
ing18_N2 -.-> CHEBI_17997
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20.75 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.36 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.54 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
9.47 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.6 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.69 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
12 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaF
F.Na 41.988 g/mol
|
3 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
KBr
Br.K 119.002 g/mol
|
85 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
LiCl
Cl.Li 42.394 g/mol
|
1 G_PER_L |
CHEBI:48607
lithium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
34 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 | yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 18 |
sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.55 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 19 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace element solution (see Medium [M1205])
Dose: 1 G_PER_L
Cross-reference to Medium M1205
Selenite--tungstate solution (see Medium [M431])
Dose: 0.4 G_PER_L
Cross-reference to Medium M431
Trace vitamins solution (see Medium [M701])
Dose: 2 G_PER_L
Cross-reference to Medium M701
5% Na2S・9H2O solution
Dose: 2.5 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
5% L--Cysteine・HCl・H2O solution
Dose: 2.5 G_PER_L
Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.130182Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1206 |
| 2026-01-28T19:48:11.838706Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:19.518373+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:19:51.020724Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007733 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.430595+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sulfate-Free Metako Medium" to "sulfate_free_metako_medium" for consistent matching |
| 2026-05-16T09:03:08.437521+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.328089+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.218365+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |