Overview
- External ontology
- TOGO:M1212 — Halobacteria Medium (pH 8.0)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- TOGO M1212 prepares Halobacteria Medium from TOGO M159/JCM Medium 168 and adjusts the complete medium to pH 8.0. These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. The original JCM1132 is unavailable; this record is supported by its own TOGO M1212 source and the explicitly referenced M159/J168 base.
Ingredients (0)
No ingredients recorded.
Preparation
- Adjust pH to 8.0.
Solutions
Halobacteria medium (M159)
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Casamino acids (BD-Difco) | 5 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| Yeast extract (BD-Difco) | 5 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| Sodium glutamate | 1 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 1.0 g in the source preparation brought to 1.0 L gives 1 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| Trisodium citrate | 3 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 3.0 g in the source preparation brought to 1.0 L gives 3 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| MgSO4 x 7 H2O | 20 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 20.0 g in the source preparation brought to 1.0 L gives 20 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| KCl | 2 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 2.0 g in the source preparation brought to 1.0 L gives 2 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| NaCl | 200 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 200.0 g in the source preparation brought to 1.0 L gives 200 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| FeCl2 x 4 H2O | 0.036 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 36.0 mg in the source preparation brought to 1.0 L gives 0.036 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Existing g/L value is correct: convert mg to g by dividing by 1000, then divide by the explicit 1 L prepared-base volume. These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| MnCl2 x 4 H2O | 0.00036 G_PER_L | TOGO M159 and JCM Medium 168 list this component for Halobacteria Medium; TOGO M1212 adjusts the prepared medium to pH 8.0. 0.36 mg in the source preparation brought to 1.0 L gives 0.00036 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Existing g/L value is correct: convert mg to g by dividing by 1000, then divide by the explicit 1 L prepared-base volume. These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
| distilled water | Water makes the preparation up to 1.0 L. Its separate input volume and mass are unspecified; neither 1 g/L nor a fixed 1 L water dose is supported. These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified. |
These concentrations describe the prepared J168/M159 base, not an asserted post-titration final-medium concentration. The recipe uses 1 L of prepared base and then adjusts pH to 8.0. The titrant identity, dose and resulting final volume are unspecified; do not infer a carbonate reagent, zero dose, or final-medium dilution factor. Existing chemical mappings and roles are preserved, not independently recertified.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.144309Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1212 |
| 2026-03-09T08:04:15.987866+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:51.083305Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007740 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:09.497821+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halobacteria Medium (pH 8.0)" to "halobacteria_medium_ph_8_0" for consistent matching |
| 2026-05-16T09:03:08.505874+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_archaea_togo_score40.py | RESOLVED_ARCHAEA_TOGO_SCORE40_GRAPH | TOGO M1212 prepares Halobacteria Medium from TOGO M159/JCM Medium 168 and adjusts the complete medium to pH 8.0. |
| 2026-10-09T14:23:51.976655+00:00 | codex | CURATED_HALOBACTERIA_VARIANT_CONCENTRATIONS_AND_PREPARATION_STAGES | Source-specific ingredient and variant review. Every change has an exact PURL-backed excerpt. Prepared-base quantities, unquantified water/titrant, source limitations, chemical identities and prior history are preserved; no taxa-media assertions are added. |