neutral_oligotrophic_haloarchaeal_medium

CultureMech:007747 archaea COMPLEX SOLID_AGAR
Original name: Neutral Oligotrophic Haloarchaeal Medium

Overview

External ontology
TOGO:M1219 — Neutral Oligotrophic Haloarchaeal Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1219 Original source: JCM - JCM_M1138-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1138

Composition

flowchart LR
    CultureMech_007747["neutral_oligotrophic_haloarchaeal_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007747 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NH4Cl["NH4Cl"]
    CultureMech_007747 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_007747 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007747 --> ing3_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing3_Sodium_pyruvate -.-> CHEBI_50144
    ing4_agar["agar"]
    CultureMech_007747 --> ing4_agar
    CHEBI_2509["CHEBI:2509"]
    ing4_agar -.-> CHEBI_2509
    ing5_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
    CultureMech_007747 --> ing5_Yeast_extract__Oxoid_
    ing6_Peptone["Peptone"]
    CultureMech_007747 --> ing6_Peptone

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
233 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component
2 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
3 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1 G_PER_L CHEBI:50144
sodium pyruvate
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
5 agar 20 G_PER_L CHEBI:2509
agar
Role: Solidifying component
6 Yeast extract (Oxoid) 0.05 G_PER_L Properties: Undefined component, Complex component
7 Peptone 0.25 G_PER_L

Solutions

MDS salt water (see Medium [M578])

Dose: 767 G_PER_L

Cross-reference to Medium M578

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.155778Z togo-import Imported from TOGO Medium Source: JCM, ID: M1219
2026-01-28T19:48:20.538132Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:34.017210+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:51.134172Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007747
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.549227+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Neutral Oligotrophic Haloarchaeal Medium" to "neutral_oligotrophic_haloarchaeal_medium" for consistent matching
2026-05-16T09:03:08.562624+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:31.457601+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.270100+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.