mollicutes_h_rf3_medium

CultureMech:007771 bacterial COMPLEX LIQUID
Original name: Mollicutes H-RF3 Medium

Overview

External ontology
TOGO:M1241 — Mollicutes H-RF3 Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1241 Original source: JCM - JCM_M1159 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1159

Composition

flowchart LR
    CultureMech_007771["mollicutes_h_rf3_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007771 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007771 --> ing1_Yeast_extract
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007771 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007771 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007771 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_007771 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007771 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_007771 --> ing7_Casamino_acids__BD_Difco_
    ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007771 --> ing8_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing8_Carbon_dioxide_gas -.-> CHEBI_16526
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007771 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.54 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound
6 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8 Casamino acids (BD-Difco) 1 G_PER_L Properties: Undefined component
9 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
10 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace minerals (see Medium [M142])

Dose: 1 G_PER_L

Cross-reference to Medium M142

8% NaHCO3 solution*

Dose: 30 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

5% Na2S・9H2O solution

Dose: 6 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

5% L-Cysteine・HCl・H2O solution

Dose: 6 G_PER_L

Properties: Defined component, Organic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.203650Z togo-import Imported from TOGO Medium Source: JCM, ID: M1241
2026-01-28T19:48:22.963523Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:38.166690+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:19:51.335207Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007771
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.757600+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Mollicutes H-RF3 Medium" to "mollicutes_h_rf3_medium" for consistent matching
2026-05-16T09:03:08.763124+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.665967+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.351600+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.