Overview
- External ontology
- TOGO:M1249 — MMFC1 Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1249 Original source: JCM - JCM_M1167 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1167
Composition
flowchart LR
CultureMech_007779["mmfc1_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007779 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007779 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_007779 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_007779 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007779 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_007779 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_007779 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_K2HPO4["K2HPO4"]
CultureMech_007779 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Resazurin["Resazurin"]
CultureMech_007779 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_N2["N2"]
CultureMech_007779 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
ing10_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007779 --> ing10_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_2H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_007779 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007779 --> ing12_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_7H2O -.-> CHEBI_75836
ing13_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007779 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_007779 --> ing14_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2_6H2O -.-> CHEBI_35696
ing15_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_007779 --> ing15_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_6H2O -.-> CHEBI_34887
ing16_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_007779 --> ing16_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_2H2O -.-> CHEBI_86318
ing17_ZnCl2["ZnCl2"]
CultureMech_007779 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_007779 --> ing18_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_5H2O -.-> CHEBI_131361
ing19_Na2_EDTA["Na2-EDTA"]
CultureMech_007779 --> ing19_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing19_Na2_EDTA -.-> CHEBI_64734
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
246.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.5, 246.0] |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
2.834 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.25, 0.584] |
| 5 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
14.025 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.025, 14.0] |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.227 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.348 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 11 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.944 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
3.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.257 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
1.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
1.19 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.205 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.136 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.395 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
22.59 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Trace mineral solution (see below)
Dose: 1 G_PER_L
50% (v/v) Methanol solution
Dose: 8 G_PER_L
Role: Solvating media; Properties: Defined component, Organic compound, Solution
5% L-Cysteine・HCl・H2O solution
Dose: 0.35 G_PER_L
Properties: Defined component, Organic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 20 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.215533Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1249 |
| 2026-01-28T19:48:38.395918Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:04.253052+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:36.211330Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:51.394979Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007779 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.816131+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMFC1 Medium" to "mmfc1_medium" for consistent matching |
| 2026-05-16T09:03:08.818727+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.725356+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.217121+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.375169+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:43.550859+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |