Overview
- External ontology
- TOGO:M124 — Medium 10
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M124 Original source: JCM - JCM_M133 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=133
Composition
flowchart LR
CultureMech_007780["medium_10"]
ing0_Distilled_water["Distilled water"]
CultureMech_007780 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Cellobiose["Cellobiose"]
CultureMech_007780 --> ing1_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing1_Cellobiose -.-> CHEBI_17057
ing2_Glucose["Glucose"]
CultureMech_007780 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Soluble_starch["Soluble starch"]
CultureMech_007780 --> ing3_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing3_Soluble_starch -.-> CHEBI_28017
ing4_Agar["Agar"]
CultureMech_007780 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007780 --> ing5_Yeast_extract__BD_Difco_
ing6_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_007780 --> ing6_Trypticase_peptone__BD_BBL_
ing7_DL_____Methyl_butyric_acid["DL--α--Methyl butyric acid"]
CultureMech_007780 --> ing7_DL_____Methyl_butyric_acid
ing8_n__Butyric_acid["n--Butyric acid"]
CultureMech_007780 --> ing8_n__Butyric_acid
ing9_Acetic_acid["Acetic acid"]
CultureMech_007780 --> ing9_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing9_Acetic_acid -.-> CHEBI_15366
ing10_n__Valeric_acid["n--Valeric acid"]
CultureMech_007780 --> ing10_n__Valeric_acid
ing11_iso__Valeric_acid["iso--Valeric acid"]
CultureMech_007780 --> ing11_iso__Valeric_acid
ing12_iso__Butyric_acid["iso--Butyric acid"]
CultureMech_007780 --> ing12_iso__Butyric_acid
ing13_Propionic_acid["Propionic acid"]
CultureMech_007780 --> ing13_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing13_Propionic_acid -.-> CHEBI_30768
ing14_K2HPO4["K2HPO4"]
CultureMech_007780 --> ing14_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing14_K2HPO4 -.-> CHEBI_131527
ing15_NaCl["NaCl"]
CultureMech_007780 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
ing16_KH2PO4["KH2PO4"]
CultureMech_007780 --> ing16_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing16_KH2PO4 -.-> CHEBI_63036
ing17__NH4_2SO4["(NH4)2SO4"]
CultureMech_007780 --> ing17__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing17__NH4_2SO4 -.-> CHEBI_62946
ing18_CaCl2["CaCl2"]
CultureMech_007780 --> ing18_CaCl2
CHEBI_3312["CHEBI:3312"]
ing18_CaCl2 -.-> CHEBI_3312
ing19_MgSO4_H2O["MgSO4・H2O"]
CultureMech_007780 --> ing19_MgSO4_H2O
CHEBI_32599["CHEBI:32599"]
ing19_MgSO4_H2O -.-> CHEBI_32599
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1050.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 850.0, 100.0, 100.0] |
| 2 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 4 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 5 | Agar | 18 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 7 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component | |
| 8 | DL--α--Methyl butyric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 9 | n--Butyric acid | 4 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 10 |
Acetic acid
C2H4O2 60.052 g/mol
|
17 G_PER_L |
CHEBI:15366
acetic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 11 | n--Valeric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 12 | iso--Valeric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 13 | iso--Butyric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 14 |
Propionic acid
C3H6O2 74.079 g/mol
|
6 G_PER_L |
CHEBI:30768
propionic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 15 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.78 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
1.18 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.47 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.12 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
MgSO4・H2O
Mg.O4S 120.368 g/mol
|
0.25 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
0.1% Resazurin solution
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
0.2% Hemin solution
Dose: 1 G_PER_L
Role: Growth factor; Properties: Organic compound, Defined component, Solution
4% Na2SO3 solution
Dose: 100 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
10% Toray silicone SH 5535 solution
Dose: 5 G_PER_L
Role: Defoaming agent; Properties: Defined component, Solution
25% L--Ascorbic acid solution
Dose: 2 G_PER_L
Role: Vitamin; Properties: Organic compound, Solution, Defined component
5% L--Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
VFA solution (see below)
Dose: 3.1 G_PER_L
Salt solution No. 1 (see below)
Dose: 37.5 G_PER_L
Salt solution No. 2 (see below)
Dose: 37.5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.198082Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M124 |
| 2026-01-28T19:48:18.282090Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:30.294639+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T03:08:59.251194Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:51.408258Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007780 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.829585+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium 10" to "medium_10" for consistent matching |
| 2026-05-16T09:03:08.833283+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.742309+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.381136+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |