clostridum_g4_medium (CultureMech:007782)

Overview

External ontology

TOGO:M1251 — Clostridum G4 Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M1251 Original source: JCM - JCM_M1169 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1169

Composition

flowchart LR
    CultureMech_007782["clostridum_g4_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007782 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007782 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007782 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007782 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007782 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_007782 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Glucose["Glucose"]
    CultureMech_007782 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Sodium_ascorbate["Sodium ascorbate"]
    CultureMech_007782 --> ing7_Sodium_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing7_Sodium_ascorbate -.-> CHEBI_113451
    ing8_KOH["KOH"]
    CultureMech_007782 --> ing8_KOH
    CHEBI_32035["CHEBI:32035"]
    ing8_KOH -.-> CHEBI_32035
    ing9_N2["N2"]
    CultureMech_007782 --> ing9_N2
    CHEBI_17997["CHEBI:17997"]
    ing9_N2 -.-> CHEBI_17997

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	MgSO4・7H2O 7H2O.Mg.O4S 246.473 g/mol	0.1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3	NaCl Cl.Na 58.443 g/mol	5 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.7 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
5	NH4Cl Cl.H4N 53.492 g/mol	0.5 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6	K2HPO4 HO4P.2K 174.174 g/mol	0.7 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7	Glucose C6H12O6 180.156 g/mol	2 G_PER_L	CHEBI:17234 glucose	Role: Carbon source; Properties: Simple component, Organic compound, Defined component
8	Sodium ascorbate C6H7O6.Na 198.106 g/mol	1 G_PER_L	CHEBI:113451 sodium ascorbate	Properties: Defined component, Organic compound, Simple component
9	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	Role: Mineral source; Properties: Defined component, Solution
10	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: Gas

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.222213Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1251
2026-01-28T19:48:30.558887Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: KOH; Added default concentration for ingredient: N2
2026-03-13T06:19:51.421651Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007782
2026-03-17T06:30:30.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.844742+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Clostridum G4 Medium" to "clostridum_g4_medium" for consistent matching
2026-05-16T09:03:08.847408+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:31.758406+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.241002+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.388488+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.