Overview
- External ontology
- TOGO:M1252 — A7 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1252 Original source: JCM - JCM_M1170 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1170
Composition
flowchart LR
CultureMech_007783["a7_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007783 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007783 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_007783 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_007783 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_007783 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_007783 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_Cellobiose["Cellobiose"]
CultureMech_007783 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007783 --> ing7_Yeast_extract__BD_Difco_
ing8_Urea["Urea"]
CultureMech_007783 --> ing8_Urea
CHEBI_16199["CHEBI:16199"]
ing8_Urea -.-> CHEBI_16199
ing9_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_007783 --> ing9_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing10_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007783 --> ing10_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_6H2O -.-> CHEBI_86345
ing11_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007783 --> ing11_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_7H2O -.-> CHEBI_75836
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
37.51 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 0.01, 37.5] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 8 | Yeast extract (BD-Difco) | 6 G_PER_L | Properties: Undefined component | |
| 9 |
Urea
CH4N2O 60.056 g/mol
|
2.1 G_PER_L |
CHEBI:16199
urea
|
Properties: Simple component, Defined component, Organic compound |
| 10 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 11 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
25 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 12 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Mineral solution (see below)
Dose: 0.2 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.223556Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1252 |
| 2026-01-28T19:48:35.025492Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:58.180895+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:29.829902Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:51.428798Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007783 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.852717+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A7 Medium" to "a7_medium" for consistent matching |
| 2026-05-16T09:03:08.856012+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.767104+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.392256+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:43.584161+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |