Overview
- External ontology
- TOGO:M1254 — M39 Methylomonas Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1254 Original source: JCM - JCM_M1171-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1171
Composition
flowchart LR
CultureMech_007785["m39_methylomonas_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007785 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007785 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007785 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NaNO3["NaNO3"]
CultureMech_007785 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_K2SO4["K2SO4"]
CultureMech_007785 --> ing4_K2SO4
CHEBI_32036["CHEBI:32036"]
ing4_K2SO4 -.-> CHEBI_32036
ing5_agarose["agarose"]
CultureMech_007785 --> ing5_agarose
CHEBI_2511["CHEBI:2511"]
ing5_agarose -.-> CHEBI_2511
ing6_H2SO4["H2SO4"]
CultureMech_007785 --> ing6_H2SO4
CHEBI_26836["CHEBI:26836"]
ing6_H2SO4 -.-> CHEBI_26836
ing7_methane["methane"]
CultureMech_007785 --> ing7_methane
CHEBI_16183["CHEBI:16183"]
ing7_methane -.-> CHEBI_16183
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.099 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.147 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
0.17 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
K2SO4
2K.O4S 174.259 g/mol
|
0.349 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
agarose
(C12H18O9)n.C12H20O10 630.549 g/mol
|
15 G_PER_L |
CHEBI:2511
agarose
|
Role: Solidifying component |
| 7 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 8 |
methane
CH4 16.043 g/mol
|
variable VARIABLE |
CHEBI:16183
methane
|
Properties: Gas |
Solutions
1.0 M NaHCO3 solution*
Dose: 3 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1.0 M KH2PO4 solution
Dose: 1 G_PER_L
Role: Buffer; Properties: Defined component, Solution, Inorganic compound
0.2 M CeCl3 solution*
Dose: 0.1 G_PER_L
Role: Mineral source
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
Trace element solution (see Medium [M1253])
Dose: 1 G_PER_L
Cross-reference to Medium M1253
Vitamins solution (see Medium [M1253])
Dose: 0.1 G_PER_L
Cross-reference to Medium M1253
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.228712Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1254 |
| 2026-01-28T19:48:36.687938Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: methane |
| 2026-03-09T08:05:01.222096+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:19:51.450901Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007785 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.876608+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M39 Methylomonas Medium" to "m39_methylomonas_medium" for consistent matching |
| 2026-05-16T09:03:08.880807+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.792935+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.273017+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.403451+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:17.660843+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |