Overview
- External ontology
- TOGO:M1264 — Cellulolytic Natronoarchaea Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1264 Original source: JCM - JCM_M1180 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1180
Composition
flowchart LR
CultureMech_007796["cellulolytic_natronoarchaea_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007796 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007796 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HPO4["K2HPO4"]
CultureMech_007796 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KCl["KCl"]
CultureMech_007796 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaHCO3["NaHCO3"]
CultureMech_007796 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2CO3["Na2CO3"]
CultureMech_007796 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
128 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.75 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
2.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
15 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
95 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
Solutions
10% Yeast extract solution
Dose: 0.2 G_PER_L
Role: Nutrient source; Properties: Undefined component
10% NH4Cl solution
Dose: 1.25 G_PER_L
Role: Nitrogen source; Properties: Defined component, Solution, Inorganic compound
1 M MgSO4 solution
Dose: 1 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound
10% Cellobiose solution
Dose: 20 G_PER_L
Role: Carbon source; Properties: Organic compound, Solution, Defined component
Trace element solution (see Medium [M1148])
Dose: 1 G_PER_L
Cross-reference to Medium M1148
Trace vitamins (see Medium [M190])
Dose: 5 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.248274Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1264 |
| 2026-01-28T19:48:27.964439Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:46.270206+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:19:51.537753Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007796 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.964772+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Cellulolytic Natronoarchaea Medium" to "cellulolytic_natronoarchaea_medium" for consistent matching |
| 2026-05-16T09:03:08.964292+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.881495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.439058+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |