Overview
- External ontology
- TOGO:M1265 — Modified Cellulolytic Haloarchaea Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- JCM Medium 1181 liquid Modified Cellulolytic Haloarchaea Medium uses MDS salt water and Potassium phosphate buffer from JCM 574, JCM 1079 Trace element solution, JCM 197 Trace vitamins, NH4Cl, cellobiose, and a Na2CO3 pH-adjustment stock.
Composition
flowchart LR
CultureMech_007797["modified_cellulolytic_haloarchaea_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007797 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007797 --> ing1_Yeast_extract__BD_Difco_
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
124.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1181 brings the MDS salt water, trace elements, and yeast extract to 958 ml with distilled water. |
| 2 | Yeast extract (BD-Difco) | 0.1 G_PER_L | JCM Medium 1181 lists 0.1 g/L Yeast extract (BD-Difco). |
Preparation
- Combine MDS salt water, JCM 1079 Trace element solution, yeast extract, and distilled water.
- Autoclave the base medium before adding sterile NH4Cl, phosphate, vitamin, and cellobiose stocks.
- Readjust pH to 7.0 with sterilized 10% Na2CO3 solution if necessary.
Solutions
MDS salt water
Dose: 833.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 240.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| MgCl2 x 6H2O | 30.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| MgSO4 x 7H2O | 35.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| KCl | 7.0 G_PER_L | JCM Medium 574 prints this component in its stock solution. |
| 1 M CaCl2 solution | 5.0 ML_PER_L | JCM Medium 574 adds 5.0 ml/L 1 M CaCl2 solution to MDS salt water. |
| Distilled water | 1.0 L | JCM Medium 574 prints this component in its stock solution. |
JCM Medium 1181 adds 833.0 ml/L MDS salt water from JCM Medium 574.
Trace element solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA | 5.0 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| FeSO4 x 7H2O | 2.0 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| ZnSO4 x 7H2O | 0.10 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| MnCl2 x 4H2O | 0.03 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| H3BO3 | 0.3 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| CoCl2 x 6H2O | 0.2 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| CuCl2 x 2H2O | 0.01 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| NiCl2 x 6H2O | 0.02 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| Na2MoO4 x 2H2O | 0.03 G_PER_L | JCM Medium 1079 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 1079 prints this component in its stock solution. |
JCM Medium 1181 adds 1.0 ml/L Trace element solution from JCM Medium 1079.
1 M NH4Cl
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NH4Cl | 1.0 MOLAR | JCM Medium 1181 identifies this as a 1.0 M stock solution. |
JCM Medium 1181 adds 5.0 ml/L 1 M NH4Cl after autoclaving.
Potassium phosphate buffer
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| 1 M K2HPO4 | 417.0 ML_PER_L | JCM Medium 574 prepares Potassium phosphate buffer from 83.4 volumes of 1 M K2HPO4, 16.6 volumes of 1 M KH2PO4, and an equal volume of distilled water. |
| 1 M KH2PO4 | 83.0 ML_PER_L | JCM Medium 574 prepares Potassium phosphate buffer from 83.4 volumes of 1 M K2HPO4, 16.6 volumes of 1 M KH2PO4, and an equal volume of distilled water. |
| Distilled water | 500.0 ML_PER_L | JCM Medium 574 prints this component in its stock solution. |
JCM Medium 1181 adds 2.0 ml/L Potassium phosphate buffer from JCM Medium 574.
Trace vitamins
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 197 prints this component in its stock solution. |
JCM Medium 1181 adds 5.0 ml/L Trace vitamins from JCM Medium 197.
0.2 M Cellobiose solution
Dose: 30.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Cellobiose | 0.2 MOLAR | JCM Medium 1181 identifies this as a 0.2 M stock solution. |
JCM Medium 1181 adds 30.0 ml/L 0.2 M Cellobiose solution after autoclaving.
10% Na2CO3 solution
Dose: variable ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 10 PERCENT_W_V | JCM Medium 1181 identifies this as a 10% w/v stock solution. |
JCM Medium 1181 readjusts pH to 7.0 with sterilized 10% Na2CO3 solution if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.249966Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1265 |
| 2026-01-28T19:48:09.929653Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 solution |
| 2026-03-09T08:04:16.140280+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:19:51.544058Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007797 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.971605+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Cellulolytic Haloarchaea Medium" to "modified_cellulolytic_haloarchaea_medium" for consistent matching |
| 2026-05-16T09:03:08.971838+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.889107+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | JCM Medium 1181 liquid Modified Cellulolytic Haloarchaea Medium uses MDS salt water and Potassium phosphate buffer from JCM 574, JCM 1079 Trace element solution, JCM 197 Trace vitamins, NH4Cl, cellobiose, and a Na2CO3 pH-adjustment stock. |